Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for purifying acarbose from acarbose-containing solution

A kind of acarbose and solution technology, which is applied in the field of purifying acarbose from acarbose-containing solution, and achieves the effects of improving the total yield, being suitable for large-scale industrial production, and being easy to operate.

Inactive Publication Date: 2015-06-10
CHENGDU UNIV
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the neutralization process, neutralization with alkaline solution or static neutralization with weak alkaline resin (in the resin treatment method, the resin damage rate is high and the batch consumption is large), both are prone to local over-alkali, resulting in abnormality of component A Increase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for purifying acarbose from acarbose-containing solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare the fermented liquid that contains 304.0 grams of acarbose, wherein A impurity content 0.85% (external standard method, relative to acarbose), adjust pH 4.0 with oxalic acid, obtain the filtrate that contains acarbose by plate and frame filtration, pass Equipped with DK110 (H + type) ion exchange column, the flow rate is controlled at 1.0 times the column volume / hour. 3 -2 type) weakly basic resin column to neutralize to pH 4.6, and neutralize the solution through CT151 strong acid resin (H + Type) column ion exchange column for rough extraction, the flow rate is controlled at 1.3 times the column volume / hour, after the column is loaded, it is eluted with 0.4mol / L hydrochloric acid, the effluent containing acarbose is collected, and the D301 (CO 3 -2 type) Weakly basic resin to neutralize to pH 4.8 and then pass the neutralization solution through PT151 (H + type) strong acid resin column ion exchange column for chromatography, the flow rate is controlled at...

Embodiment 2

[0024] Prepare the fermented liquid that contains 250.4 grams of acarbose, wherein A impurity content 0.98% (external standard method, with respect to acarbose), adjust pH 5.0 with 40% lactic acid (v / v), plate frame filtration obtains containing Acarbose The filtrate of carbose was packed with DK110 (H + type) ion-exchange column, the flow rate is controlled at 0.9 times the column volume / hour. 3 -2 type) weakly basic resin column to neutralize to pH 4.2, and neutralize the solution through CT151 strong acid resin (H + Type) column ion exchange column for rough extraction, the flow rate is controlled at 1.3 times the column volume / hour, after the column is loaded, it is eluted with 0.36mol / L hydrochloric acid, the effluent containing acarbose is collected, and the A845 (CO 3 -2 type) weakly basic resin to neutralize to pH 4.7 and then pass the neutralization solution through PT151 (H + type) strong acid resin column ion exchange column for chromatography, the flow rate is ...

Embodiment 3

[0026] Prepare a crude product solution containing 150.4 g of acarbose, wherein the impurity A content is 0.75% (external standard method, relative to acarbose), adjust pH 4.0 to 5.0 with 20% (v / v) phosphoric acid, and pass the solution through CT151 strong Acid resin (H + Type) column ion exchange column for rough extraction, the flow rate is controlled at 1.6 times the column volume / hour, after the column is loaded, it is eluted with 0.48mol / L hydrochloric acid, the effluent containing acarbose is collected, and the A845 (CO 3 -2 type) weakly basic resin to neutralize to pH 4.1, and then pass the neutralization solution through PT151 (H + type) strong acid resin column ion exchange column for chromatography, the flow rate is controlled at 0.6 times the column volume / hour, after the column is loaded, it is eluted with 0.19mol / L hydrochloric acid to collect Aka containing less than 0.6% of component A Wave sugar effluent with A845 (CO3 -2 type) weakly basic resin neutrali...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for purifying acarbose from an acarbose-containing solution and aims at solving the problem of effectively controlling and reducing the content of impurities A in the acarbose production process. The neutralization of an acidic acarbose eluate is performed on a weakly alkali ion exchange column and the operation is simple and convenient; meanwhile, the abnormal increase of the component A of the acarbose due to partially excessive alkali in the process of neutralization by use of an alkaline solution or by use of a static state is avoided. The method comprises the following specific operation process: treating the acarbose-containing solution by use of a weakly acidic solution to obtain the filtrate containing the acarbose, putting the filtrate through a weak-acid ion exchange column, neutralizing by use of a weakly alkaline resin, next, separating and purifying by use of a strong acidic resin, and then concentrating by use of a nanofiltration membrane and performing spray drying, thereby finally obtaining the finished acarbose product having the component A content of lower than 0.6%. The method is simple in separation process; besides, the component A content of the crude product of the acarbose is effectively controlled, the process of independently separating the component A is avoided and the total yield is greatly increased; in short, the method is suitable for industrialized production.

Description

Technical field: [0001] The present invention relates to a method for producing acarbose, in particular to a method for producing acarbose with a low A-component content from a solution containing acarbose. Background technique: [0002] Acarbose (Acarbose ) is an α-glucose inhibitor, which can be taken orally to control the postprandial blood sugar rise in patients with type II diabetes. Acarbose produced by Actinomycetes uthaensis ( Actinoplanes utahensis) produced by fermentation, separated and purified by ion exchange resin. In the industrial production of acarbose, impurity A (impurity A) is an isomer of acarbose, the structure and properties are very close to acarbose, it is difficult to separate through ion exchange chromatography, 2010 edition The Chinese Pharmacopoeia stipulates that the impurity should not exceed 1.5%. The United States Pharmacopoeia USP35-NF30, European Pharmacopoeia EP8.0 and British Pharmacopoeia BP201 all require that the content of impu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/06
CPCC07H1/06C07H15/203
Inventor 王欣荣杨渊杨赞李旭航孙敏翟龙飞徐明琴
Owner CHENGDU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com