Metaplexis japonica C21 steroid extractive, and preparation method and application of metaplexis japonica C21 steroid extractive
A technology of extract and radish, which is applied in the field of medicine, can solve the problems of no research reports on tumor cell multidrug resistance reversal agents, no high-content radish C21 steroid extract products, etc.
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Embodiment 1
[0057] Method for Determination of C21 Steroid Weight Percentage in Radix Radix C21 Steroid Extract by Colorimetry
[0058] Solution preparation
[0059] Preparation of reference solution
[0060] Accurately weigh an appropriate amount of nodolimonin reference substance, add methanol to make each 1 ml containing 198.72 mu g solution, as the reference solution.
[0061] Preparation of the test solution
[0062] Take 0.1 g of radish C21 steroid extract, weigh it accurately, add methanol to dissolve, transfer to a 50 ml measuring bottle, add methanol to the mark, shake well, accurately draw 10 ml, put it in a 100 ml measuring bottle, add methanol to scale, shake well, filter, and take the continued filtrate as the test solution.
[0063] Assay method
[0064] Precisely draw the test solution 100 mu l, put it in a stoppered test tube, evaporate the solvent, add 5 ml of freshly prepared concentrated sulfuric acid-methanol (6:1) precisely, shake well, put it ...
Embodiment 2
[0066] Take 100 Kg of dried cane powder of radish, add 8 times the amount of water to reflux and extract 3 times, each time for 2 hours, filter, combine the extracts, recover ethanol under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to the HPD100 macroporous adsorption resin column, elute with water until the eluent is nearly colorless, discard the eluent, and elute with 10% ethanol until the eluent is nearly colorless, discard the eluent Remove the liquid, elute with 90% ethanol until the eluate is nearly colorless, collect the eluate, add the 90% ethanol eluate to the D941 decolorization resin column, elute with 6 times the column volume of 90% ethanol, collect the eluate liquid, concentrated under reduced pressure, and dried to obtain 1.47 Kg of Radix Radix C21 steroid extract, wherein the weight percentage of C21 steroid is 52%.
Embodiment 3
[0068] Take 100 Kg of dried cane powder of radish, add 8 times the amount of 30% ethanol to reflux and extract 3 times, each time for 2 hours, filter, combine the extracts, and recover the ethanol under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to HPD300 macroporous adsorption resin column, elute with water until the eluent is nearly colorless, discard the eluent, and elute with 50% ethanol until the eluent is nearly colorless, discard the eluent Remove the liquid, elute with 60% ethanol until the eluate is almost colorless, collect the eluate, add the 60% ethanol eluate to the D941 decolorization resin column, elute with 6 times the column volume of 90% ethanol, collect the eluate liquid, concentrated under reduced pressure, and dried to obtain 0.46 Kg of Radix Radix C21 steroid extract, wherein the weight percentage of C21 steroid is 91%.
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