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Phyllostachys pubescens tissue culture method

A tissue culture and bamboo technology, applied in the field of plant tissue culture, can solve the problems of difficulty in obtaining materials, low reproduction coefficient, and difficulty in controlling the cost at a low level, and achieves the effects of strong reproduction, high reproduction coefficient and large income.

Inactive Publication Date: 2015-06-24
罗焕荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional moso bamboo propagation methods are mainly based on asexual reproduction methods such as burying whips, burying stalks, and burying joints. However, traditional vegetative propagation methods are difficult to obtain materials, have low reproduction coefficients, and are difficult to control at a low level. It is difficult to meet the needs of current production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Seed germination: The collected seeds are dried in a ventilated and cool place, and then placed in a refrigerator at 4°C for sealed storage for later use. Remove the lemma of the seeds, select the complete seeds with full grains and bright color and remove the hair tips. After washing away the surface attachments with clean water, soak in laundry detergent for 5 minutes, and then rinse with running water for 1 hour. After soaking the seeds at 30°C for 10 hours, disinfect them with 4% sodium hypochlorite solution for 10 minutes in an ultra-clean workbench, then wash them with sterile water for 3 times, then disinfect them with 0.1% mercuric solution for 10 minutes, wash them with sterile water for 4 times, and then wash them with sterile water for 10 minutes. Bacteria filter paper was inoculated into the seed germination medium in a flat manner after absorbing water to induce culture. After inoculation, they were first cultured in total darkness at 25°C for 7 da...

Embodiment 2

[0021] (1) Seed germination: The collected seeds are dried in a ventilated and cool place, and then placed in a refrigerator at 4°C for sealed storage for later use. Remove the lemma of the seeds, select the complete seeds with full grains and bright color and remove the hair tips. After washing away the surface attachments with clean water, soak in laundry detergent for 10 minutes, then rinse with running water for 3 hours. After soaking the seeds at 30°C for 10 hours, disinfect them with 4% sodium hypochlorite solution for 20 minutes in an ultra-clean workbench, then wash them with sterile water for 5 times, then disinfect them with 0.2% mercuric solution for 15 minutes, rinse them with sterile water for 5 times, and then wash them with sterile water. Bacteria filter paper was inoculated into the seed germination medium in a flat manner after absorbing water to induce culture. After inoculation, they were first cultured in total darkness at 28°C for 12 days, and then pl...

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Abstract

The invention discloses a phyllostachys pubescens tissue culture method. Phyllostachys pubescens is also called as mao bamboo, belongs to gramineous phyllostachys plants, is a bamboo species which integrates economic benefits, ecological functions and ornamental values, and has the characteristics of rapidness in growth, strong propagation, early maturing, wide application, low investment and large profit; and tissue culture seedling raising has the characteristics of rapid propagation, less cost and high multiplication coefficient; and according to the phyllostachys pubescens tissue culture method disclosed by the invention, phyllostachys pubescens seeds are taken as explants, a phyllostachys pubescens tissue culture rapid propagation technology system is established by virtue of steps such as seed disinfection, induction, multiplication, rooting and acclimatization and transplanting, and the phyllostachys pubescens tissue culture rapid propagation technology system can be used for providing technological support for further industrialized seedling raising of gramineous phyllostachys tissue culture, and can also be used for laying a foundation for genetic transformation and species improvement of phyllostachys pubescens.

Description

technical field [0001] The invention relates to a plant tissue culture method in agricultural biotechnology, in particular to a moso bamboo tissue culture method. Background technique [0002] bamboo( Phyllostachys Pubescens ), also known as bamboo, belongs to the genus Bambusa in the Poaceae family. It is a large-scale perennial evergreen scattered bamboo. It has the characteristics of fast growth, strong reproduction, early maturity, wide application, low investment, and large returns. my country is one of the central distribution areas of moso bamboo, known as "East Asian civilization and bamboo civilization", mainly distributed in the south of the Yangtze River, north of Nanling, west of Daiyun Mountain, and east of Wuling Mountain. Moso bamboo forest plays an important role in agricultural construction while creating a good ecological environment, and also occupies a certain proportion in the national economy. The utilization and application of moso bamboo spreads ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 罗焕荣
Owner 罗焕荣
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