Method for recovering ganoderma lucidum solid strain into liquid strain

A technology of solid bacteria and liquid bacteria, applied in the biological field, can solve the problems of complex operation, long cycle, and many links, and achieve the effect of simple process, low investment and low risk

Inactive Publication Date: 2015-07-22
黄秀英
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Problems solved by technology

[0014] The production of Ganoderma lucidum liquid strains by the above method not only involves many links, complicated operation, but also a long cycle.
At the same time, professional fermentation irrigation is required to implement
Big investment, high risk

Method used

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Embodiment Construction

[0030] Below in conjunction with embodiment the method of the present invention is further described.

[0031] A kind of ganoderma lucidum solid bacterial classification is reduced to liquid bacterial classification, and the specific implementation method is as follows:

[0032] 1. Preparation of culture medium: The raw material is composed of 50% corn flour and 50% sawdust. Mix the two raw materials evenly, and then add appropriate amount of water to adjust the moisture content of the mixed material to 45-50% to obtain the culture medium .

[0033] 2. Sterilization by bottling: divide the culture medium into Erlenmeyer flasks, and the filling volume of the culture medium is one-third of that of the Erlenmeyer flasks, and then carry out disinfection and sterilization according to conventional methods.

[0034] 3. Inoculation culture: put the ganoderma strains into the triangular flask according to the conventional method, and cultivate until the ganoderma mycelium is covered ...

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Abstract

A method for recovering a ganoderma lucidum solid strain into a liquid strain particularly includes the following steps that the raw materials of a culture medium are composed of corn flour and saw dust, and the culture medium is subpackaged into a triangular bottle and then sterilized according to a common method; the ganoderma lucidum solid strain is put in the triangular bottle according to a common method, and the ganoderma lucidum mycelia is cultured to grow full of the culture medium; sterilized water is poured into the triangular bottle, the strain the bottle is smashed, and then the ganoderma lucidum solid strain is recovered into the liquid strain. A traditional ganoderma lucidum liquid strain needs a professional fermentation tank. According to the method, only the common triangular bottle is needed, investment is small, and risks are low. The safety coefficient of the method is improved by N times compared with the traditional liquid strain. In the implementation process of the method, the culture medium does not contain sugar, thereby being not prone to being polluted, and the culture medium is easy to store and convenient to convey. By means of the method, the prepared ganoderma lucidum liquid strain has the same effect as the traditional ganoderma lucidum liquid strain, but the investment is reduced by nearly 50 times.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the reduction of ganoderma lucidum solid strains to liquid strains. Background technique [0002] "Liquid strain" is a liquid form of edible fungus strain produced by submerged culture (liquid fermentation in the shape of liquid strain) technology in a biological fermenter with a liquid medium. [0003] "Liquid" refers to the physical state of the medium, and "submerged liquid culture" is the fermentation engineering technology. The essence of "liquid seed production" is to use biological fermentation engineering to produce liquid strains to replace traditional and simple solid seed production; to use the principle of biological fermentation to provide an optimal nutrient, pH, temperature, and oxygen supply for mycelia growth, so that The hyphae grow rapidly and proliferate rapidly, reaching a certain number of bacterial balls in a short time, and completing a fermentati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 黄秀英
Owner 黄秀英
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