Method for recovering ganoderma lucidum solid strain into liquid strain
A technology of solid bacteria and liquid bacteria, applied in the biological field, can solve the problems of complex operation, long cycle, and many links, and achieve the effect of simple process, low investment and low risk
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[0030] Below in conjunction with embodiment the method of the present invention is further described.
[0031] A kind of ganoderma lucidum solid bacterial classification is reduced to liquid bacterial classification, and the specific implementation method is as follows:
[0032] 1. Preparation of culture medium: The raw material is composed of 50% corn flour and 50% sawdust. Mix the two raw materials evenly, and then add appropriate amount of water to adjust the moisture content of the mixed material to 45-50% to obtain the culture medium .
[0033] 2. Sterilization by bottling: divide the culture medium into Erlenmeyer flasks, and the filling volume of the culture medium is one-third of that of the Erlenmeyer flasks, and then carry out disinfection and sterilization according to conventional methods.
[0034] 3. Inoculation culture: put the ganoderma strains into the triangular flask according to the conventional method, and cultivate until the ganoderma mycelium is covered ...
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