Establishment method of Dioscorea alata L. tissue culture regeneration system

A technology for tissue culture and method establishment, which is applied in the field of plant tissue culture, and can solve problems such as high consumption of seed materials, decreased yield, and degeneration of species

Inactive Publication Date: 2015-07-22
冯文杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the large potato is mainly asexually propagated through underground tubers, which consumes a lot of seed materials, and this self-reserved cultivation form will degrade the seed quality, reduce the quality, and reduce the yield. Therefore, it is necessary to improve the quality, increase the yield, and reduce the planting loss. , has become an urgent problem to be solved in potato production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Disinfection treatment of explants: Take young and tender stems of large potato without disease from the field on a sunny day, rinse them with tap water, and then lightly wash the explants with neutral detergent. Rinse under running water for 1 hour and place on a sterile ultra-clean workbench for later use. First use 75% alcohol to quickly sterilize for 10 seconds, then rinse with sterile water for 3 times, and then use 0.1% HgCl 2 Disinfect for 5 minutes respectively, rinse with sterile water for 3 times, and then dry the surface moisture of the explants with sterile filter paper for later use.

[0020] (2) Callus induction: inoculate the stem segment sterilized in step (1) into the germination medium for callus induction culture. After inoculation, cultivate under dark conditions, and after the seeds germinate, move them to the light for 12 hours a day, the light intensity is 1000lx, and the culture temperature is 25°C, and the relative air humidity is 75%. ...

Embodiment 2

[0026] (1) Disinfection treatment of explants: Take young and tender stems of large potato without disease from the field on a sunny day, rinse them with tap water, and then lightly wash the explants with neutral detergent. Rinse under running water for 2 hours, then place on a sterile ultra-clean workbench, and set aside. First use 75% alcohol to quickly sterilize for 15 seconds, then rinse with sterile water for 5 times, and then use 0.1% HgCl 2 Disinfect for 8 minutes respectively, rinse with sterile water for 3 times, then dry the surface moisture of the explants with sterile filter paper and set aside for later use.

[0027] (2) Callus induction: inoculate the stem segment sterilized in step (1) into the germination medium for callus induction culture. After inoculation, cultivate under dark conditions, and after the seeds germinate, move them to the light for 13 hours a day, the light intensity is 1500lx, the culture temperature is 26°C, and the relative air humidit...

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PUM

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Abstract

The invention discloses an establishment method of a Dioscorea alata L. tissue culture regeneration system. Dioscorea alata L. has an alternate name of winged yam, is a perennial twining liana and belongs to the dioscorea of the dioscoreaceae; the Dioscorea alata L. is rich in nutrition, bright in color, and excellent in taste, and is a food popular with people. The Dioscorea alata L. is drought-resistant, heat-resistant, high in yield potential, relatively high in starch content and short in growth period, and is an energy plant having great development potential. At present, the Dioscorea alata L. is mainly multiplied from underground tubes by virtue of vegetative propagation, and a great quantity of seed materials can be consumed; besides, the self-seedling cultivation form is possible to result in the degradation of the properties of the seed, the reduction of the quality and the decrease of the yield, and therefore, the establishment of the Dioscorea alata L. isolated culture regeneration system is necessary. The establishment method comprises the major process of explant sterilization treatment, inoculation into a germinating culture medium to induce the callus, callus multiplication culture, callus tissue differentiation into multiple shoots, and rooting and seedling growing culture.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for establishing a tissue culture regeneration system of large potato. Background technique [0002] Today, a large number of mining and excessive use of mineral resources have caused serious energy shortages and environmental pollution. Therefore, biomass energy based on energy plants will be an ideal choice for human beings in the future. The development of energy plants as a supplement and substitute for existing energy can not only gradually alleviate the energy crisis, but also meet the requirements of sustainable development due to low production costs. and trends. Herbaceous plants have strong adaptability to the environment, wide distribution, fast growth, and short growth cycle, which is conducive to large-scale planting and industrial production. Big potato ( Dioscorea alata L.), also known as ginseng potato, is a perennial...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冯文杰
Owner 冯文杰
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