6 results about "Dioscorea oppositifolia" patented technology

A highly-active Rhizoma dioscoreae polysaccharide and a preparation method thereof is described in relation to nutritional food production. The preparation method includes: subjecting a Rhizoma dioscoreae slurry to an ultra-high pressure treatment to produce an ultra-high pressure-treated Rhizoma dioscoreae slurry; inoculating Lactiplantibacillus plantarum subsp. plantarum into the ultra-high pressure-treated Rhizoma dioscoreae slurry, and conducting fermentation to produce a Rhizoma dioscoreae fermentation broth; and subjecting the Rhizoma dioscoreae fermentation broth to hot water extraction and then centrifugation, and collecting, concentrating, and drying a resulting supernatant to produce the Rhizoma dioscoreae polysaccharide.

The invention discloses a dioscorea bulbifera pollen in-vitro germination liquid culture medium and application thereof in pollen viability determination. The invention relates to a Dioscorea bulbifera pollen in-vitro germination liquid culture medium, which takes distilled water as a solvent and comprises the following components: polyethylene glycol PEG4000, cane sugar and boric acid. The dioscorea bulbifera pollen is collected and then dispersed in the liquid culture medium provided by the invention, observation and statistics can be carried out after the dioscorea bulbifera pollen is cultured for 3 hours in a dark place under a constant temperature condition, and the pollen activity of the dioscorea bulbifera can be effectively detected through the pollen germination rate. Compared with other existing pollen activity detection technologies, the method has the advantages of being high in accuracy, easy and convenient to operate, stable and reliable, provides important technical support for hybridization breeding and breeding biology research of the dioscorea bulbifera, is beneficial to further mining medicinal and edible wild germplasm resources of the dioscorea bulbifera, enables the dioscorea bulbifera to exert greater economic and social benefits, and has a wide application prospect. Meanwhile, beneficial reference is provided for breeding and breeding research of other economic species of the dioscorea.

This invention relates to a method for preparing and applying a fermented product of Bifida Ferment Lysate and Dioscorea Oolong with enhanced moisturizing and anti-aging effects, belonging to the field of microbial fermentation technology. This invention discloses a *Bifidobacterium longum*, the nucleotide sequence of which is shown in SEQ ID NO: 1. This invention utilizes *Bifidobacterium longum* HJ-005 to ferment *Dioscorea opposita* and *Dioscorea opposita* to prepare a *Bifida Ferment Lysate* fermented product of *Dioscorea opposita*, simultaneously increasing the content of absorbable diosgenin, anthocyanins, arginine, and organic selenium, with better effects compared to other lactic acid bacteria. It can significantly reduce the water loss and shrinkage of zebrafish embryos, exhibiting a moisturizing effect, and can significantly promote the expression of zebrafish type I collagen and Elna genes, promoting collagen and elastin regeneration, thus possessing anti-aging effects and can be used as a new raw material for cosmetics.

The invention belongs to the technical field of plant genetic engineering. The invention relates to a transcription factor, in particular to a Dioscorea composita DcAIL5 transcription factor and application thereof in increasing diosgenin content. The invention discloses the regulation and control effect of the Dioscorea composita transcription factor DcAIL5 in diosgenin biosynthesis for the first time, the accumulation of the diosgenin is obviously reduced by silencing the DcAIL5, and the accumulation of the diosgenin is obviously improved by transient overexpression. Therefore, the DcAIL5 can be used as a core target of genetic engineering and genetic improvement, is used for cultivating a new variety of Dioscorea composita with high saponin content, and is expected to relieve the supply and demand contradiction of steroid drug raw materials. The invention provides a new path for green, efficient and sustainable production of diosgenin, and has wide industrialization prospects and application values in the aspects of transgenic breeding, development of yield increasing reagents, molecular marker-assisted selection and the like.

This invention discloses a blood-stasis-removing and conditioning granule for individuals with blood stasis constitution and its preparation method. The granule comprises 5-10 parts of Dioscorea opposita, 5-10 parts of Prunus persica kernel, 5-10 parts of Hawthorn, 5-15 parts of Pueraria lobata, 5-10 parts of Angelica sinensis, 3-8 parts of Sophora japonica flower bud, 3-8 parts of Glycyrrhiza uralensis, and 2-5 parts of Rosa rugosa. This blood-stasis-removing and conditioning granule can be used to regulate the condition of individuals with blood stasis constitution. Long-term use can effectively resolve symptoms caused by blood stasis constitution, such as dull complexion, pigmentation or purplish spots, dark tongue, dark tongue or petechiae, dark or thickened sublingual veins, and a choppy pulse. It can be taken long-term as a health supplement. This blood-stasis-removing and conditioning granule can also be made into dry tablets, beverages, etc. for consumption.

The invention relates to a specific chromatogram construction method for distinguishing iron stick yam and counterfeit products thereof based on HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) and application of the specific chromatogram construction method, the iron stick yam and the counterfeit products thereof can be effectively distinguished, and the quality and the medicinal value of the iron stick yam are guaranteed. Then, detecting by using a high performance liquid chromatography electrospray detector under specific chromatographic conditions, and finally, according to a detection result, drawing a standard characteristic chromatogram of the iron stick yam medicinal material as well as the counterfeit product and the reference medicinal material of the iron stick yam medicinal material; the specific chromatogram is applied to quality detection, evaluation and control of the Tiegun yam medicinal material, or is applied to distinguishing the Tiegun yam and counterfeit products of the Tiegun yam. According to the specific chromatogram construction method, the iron stick yam and the counterfeit potatoes, cassava, sweet potatoes and dioscorea nipponica can be identified according to whether the raffinose exists in the chromatographic peak or not, the product quality and medicinal value of the iron stick yam are effectively guaranteed, and the specific chromatogram construction method has practical application value.