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The major gene tatgw‑2d controlling wheat thousand-grain weight and its marking method

A technology of major gene and labeling method, which can be used in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., and can solve the problem of unclear molecular regulation mechanism of grain weight.

Inactive Publication Date: 2018-01-05
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular regulation mechanism of grain weight is still unclear

Method used

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  • The major gene tatgw‑2d controlling wheat thousand-grain weight and its marking method
  • The major gene tatgw‑2d controlling wheat thousand-grain weight and its marking method
  • The major gene tatgw‑2d controlling wheat thousand-grain weight and its marking method

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Experimental program
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Embodiment Construction

[0035] The present invention will be described in further detail below according to the drawings and embodiments.

[0036] (1) Wheat Genomic DNA Extraction

[0037] The test materials used for DNA extraction were parental Jing 411 and Hongmangchun 21, two high-low mixed pools, recombinant inbred line RIL (Jing 411 / Hongmangchun 21) F 9 Groups of families. The specific method is:

[0038] 1. Grind the wheat single grain, take 0.1g and add 0.7mL SDS extract (0.1M Tris-HCl (PH=8.5), 0.1M NaCl, 0.05M EDTA (PH=8.0), 2% SDS) at 60°C Under cracking for 45min, during which several times of shock.

[0039]2. Centrifuge at 12000 rpm at 4°C for 10 minutes.

[0040] 3. Take the supernatant and add an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1) and rotate up and down until the two phases do not separate quickly.

[0041] 4. Centrifuge for 10 minutes at 12000 rpm at 4°C.

[0042] 5. Take the supernatant and add an equal volume of phenol: chloroform: isoamyl alcohol (2...

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Abstract

The invention discloses a main gene TaTGW‑2D for controlling the thousand-grain weight of wheat. The invention also discloses a marking method for the main effect gene TaTGW-2D for controlling the thousand-grain weight of wheat. The beneficial effect of the present invention is that, using the simplified genome sequencing technology, a candidate region closely related to wheat grain weight is identified, and the candidate gene in this region is cloned, and verified by artificial populations, the gene is a significant influence on grain weight. Novel candidate master genes and functional markers were developed (TaTGW‑2D). According to bioinformatics analysis, TaTGW‑2D contains gelsolin domain, which regulates the synthesis and decomposition of actin filaments, which play an important role in cytokinesis, organelle movement, and plasma membrane fluidity. Actin filaments affect cell growth, which may further affect grain weight. The invention provides new gene resources for high-yield breeding of wheat, and at the same time further clarifies the molecular mechanism of yield formation.

Description

technical field [0001] The invention relates to a main gene TaTGW-2D for controlling wheat thousand-grain weight and a marking method thereof, and belongs to the technical field of crop genetics and breeding. Background technique [0002] Wheat (Triticum aestivum L.) is the second largest food crop in the world. Increasing wheat production is of great strategic significance to national food security and agricultural production benefits. Thousand-grain weight is one of the three elements of wheat yield and has high heritability (Giura and Saulescu, 1996). Therefore, the breeding process of wheat is to continuously increase grain weight to a large extent. However, the molecular regulation mechanism of grain weight is still unclear. [0003] Summarizing the previous reports, thousand-grain weight is a quantitative trait, which is jointly controlled by major and minor effect polygenes. At present, there are many QTL loci related to grain weight, which are widely distributed on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 常成胡明建张海萍朱晓峰刘鹏马刚邵辉司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY