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An image processing method and device

An image processing and gray-scale image technology, applied in the field of image processing, can solve the problems of undercounting cells, omissions, etc.

Active Publication Date: 2018-06-05
SHANGHAI RUIYU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] It is impossible to accurately count the non-suspended yeast cells without obvious edges. When counting yeast cells using the methods of the prior art, it is easy to miss or undercount the number of cells.

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Embodiment Construction

[0069] The following will clearly and completely describe the technical solutions in the embodiments of the application with reference to the drawings in the embodiments of the application. Apparently, the described embodiments are only some of the embodiments of the application, not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of this application.

[0070] Before introducing the specific implementation of this application, some preparatory work needs to be performed: in general, the concentration of yeast cells in the yeast solution is relatively high. In order to observe the yeast cells under the microscope, the yeast solution needs to be diluted by a certain number of times. Then, a certain volume of yeast cell solution diluted by a certain factor is placed on the objective lens of the microscope, and an industrial camera is u...

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Abstract

The present application provides an image processing method and device, wherein one method includes: acquiring a target image containing yeast cells; performing binarization processing on the target image to obtain a target grayscale image; acquiring the target grayscale image the bright spot area in; calculate the average brightness of all pixels in each bright spot area; judge whether the average brightness of each bright spot area is greater than the first preset threshold; wherein, the first preset threshold is used to indicate whether the bright spot area is yeast Cells: taking the sum of the number of bright spot regions whose average brightness is greater than the first preset threshold as the number of yeast cells contained in the target image. This application adopts the method of counting the bright spot area, which can accurately count the number of yeast cells, thereby improving the accuracy of counting the number of yeast cells.

Description

technical field [0001] The present application relates to the technical field of image processing, and in particular to an image processing method and device. Background technique [0002] In cell analysis research in the field of biotechnology, culture dishes are generally used to cultivate cells. In order to judge the cell viability of the culture dish, it is necessary to count the number of cells in the culture dish. At present, industrial cameras are used instead of traditional microscope eyepieces to enlarge and image the cells in the culture dish, and then use image processing to calculate the number of cells in the imaging area. [0003] The method of image processing and counting in the prior art can be as follows: using the edge fitting algorithm to locate the center of the circle to identify the edge of the suspended cells in the culture dish, and count the number of the suspended cells. However, the identification method of the prior art can only be applied to t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/00
Inventor 陈睿黄海清
Owner SHANGHAI RUIYU BIOTECH