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Preparation method of cunninghamia lanceolata synthetic seed

A technology for artificial seeds and fir, which is applied in the field of plant biology to achieve the effects of reducing the process of domestication and transplanting, convenient transportation and storage, and easy operation.

Active Publication Date: 2015-07-29
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of artificial seed technology to the asexual reproduction of Chinese fir

Method used

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  • Preparation method of cunninghamia lanceolata synthetic seed
  • Preparation method of cunninghamia lanceolata synthetic seed
  • Preparation method of cunninghamia lanceolata synthetic seed

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The preparation method of Chinese fir artificial seed capsule of the present invention comprises the following steps:

[0021] (1) Cut out 0.5-1cm stem sections of fir tissue culture subculture seedlings as propagating material of fir seeds.

[0022] (2) Use ultraviolet light to irradiate for 20 minutes to sterilize the sodium alginate matrix components, CaCl 2 The solution is autoclaved.

[0023] (3) Screening of artificial seed capsule components: gelling agent sodium alginate and complexing agent CaCl in different proportions and dosages 2 The embedding material is solidified to form a seed coat capsule.

[0024] (4) Artificial seed production process: Sodium alginate is used as the embedding matrix, and the 0.5-1cm long sprout propagation material is invaded into it. After 10 minutes, the propagule is inhaled with a straw, and the complexing agent CaCl is dripped at a certain height. 2 In the solution, the embedding material is solidified, and the artificial seed...

Embodiment 2

[0030] The water retention and shaping treatment of Chinese fir artificial seed of the present invention may further comprise the steps:

[0031] (1) Cut out 0.5-1cm stem sections of fir tissue culture subculture seedlings as propagating material of fir seeds.

[0032] (2) Add 6% agar to the sodium alginate matrix components, sterilize with ultraviolet light for 20min, CaCl 2 The solution is autoclaved.

[0033] (3) Orthogonal design method was used to conduct combined experiments on calcium chloride, sodium alginate, whether to add agar, and complexation time to analyze the water retention and setting effects of Chinese fir artificial seeds.

[0034] (4) Artificial seed production process: Sodium alginate is used as the embedding matrix, and the 0.5-1cm long sprout propagation material is invaded into it. After 10 minutes, the propagule is inhaled with a straw, and the complexing agent CaCl is dripped at a certain height. 2 In the solution, the embedding material is solidif...

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Abstract

The invention discloses a preparation method of a cunninghamia lanceolata synthetic seed. The preparation method comprises the following steps: (1) using buds obtained by short stem multiplication in a cunninghamia lanceolata tissue culture process as propagules, wherein the bud multiplication culture medium is prepared from MS, 0.3 mg / L of IBA, 0.4 mg / L of 6-BA, 0.2 mg / L of VC, 30 g of sugar, 6.0 g of agar and 0.5 g of Ac; (2) preparing a seed embedding matrix with 1.5% of CaCl2, 1% of sodium alginate and agar according to complexing time of 5 minutes, after complexing, washing the seed embedding matrix with distilled water, adding 0.5% of agar into the embedding matrix, drying the water on the surface, sealing, and placing the embedding matrix into a refrigerator at the temperature of 4 DEG C. According to the preparation method disclosed by the invention, the sources of raw materials are accessible; the preparation method is simple and convenient to operate; compared with a test-tube plantlet, the cunninghamia lanceolata synthetic seed is small in size, convenient to transport and store and low in cost; the preparation method lays a foundation for popularizing and applying the cunninghamia lanceolata synthetic seed.

Description

technical field [0001] The invention relates to the field of plant biotechnology. Specifically relates to a preparation method of fir artificial seeds. Background technique [0002] Chinese fir (Cunninghamia Lanceolata) is a gymnosperm plant belonging to the family Cedaraceae and the genus Chinese fir. It is a tree with a height of more than 30 meters and a diameter at breast height of up to 3 meters. It is a unique fast-growing commercial timber tree species in my country. Warp does not crack. The material is light and tough, with moderate strength. Widely used in construction, furniture, appliances, shipbuilding and other aspects. In terms of Chinese fir breeding, with the continuous development of rapid propagation technology, many scholars have begun to pay attention to the rapid propagation technology of Chinese fir fine clones. Comparative experiments have been carried out in the areas of cultivation management and other aspects, and now the general reproduction tec...

Claims

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Application Information

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IPC IPC(8): A01C1/08
Inventor 杨梅谭玲蒋维昕徐圆圆戴勤苏治南
Owner GUANGXI UNIV
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