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A method for establishing an animal model of abnormal biliary fluid carrier UC disease by using 2,4-dinitrochlorobenzene combined with acetic acid

A technology of abnormal bile quality, dinitrochlorobenzene, applied in the direction of nitro compound active ingredient, anhydride/acid/halide active ingredient, etc. The effect of high modulus and simple modeling method

Inactive Publication Date: 2018-06-08
XINJIANG MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many abnormal body fluid syndromes and special syndromes in Uighur medicine, which mainly involve changes in a series of appearance indicators such as mental state, sleep, and tongue coating. It has been found that some characteristics and mental state are related to the disease. Although these changes cannot be used as diagnostic criteria for the disease, they are By affecting the body's neuro-endocrine-immune system, it can increase the body's susceptibility to disease

Method used

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  • A method for establishing an animal model of abnormal biliary fluid carrier UC disease by using 2,4-dinitrochlorobenzene combined with acetic acid
  • A method for establishing an animal model of abnormal biliary fluid carrier UC disease by using 2,4-dinitrochlorobenzene combined with acetic acid
  • A method for establishing an animal model of abnormal biliary fluid carrier UC disease by using 2,4-dinitrochlorobenzene combined with acetic acid

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Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Construction of an animal model of UC syndrome with abnormal bile fluid carrier

[0032] The present invention adopts Uygur medicine body fluid theory combined with 2,4-dinitrochlorobenzene compound acetic acid method to construct an animal model of abnormal bile carrier UC disease syndrome, and the specific preparation method is as follows:

[0033] (1) Experimental animals: select healthy and clean male wistar rats with a body weight of 180±30g.

[0034] (2) Feeding of experimental rats for 1-13 days: 5 rats per cage to meet the normal feeding density, and the experimental rats were given 300g of common feed per cage per day for adaptive feeding for 7 days, and then given daily feed for 7 days. Rats in the cage experiment were given 300g of common feed, 400ml of dry heat drinking water, and were continuously given biliary feed, dry heat environment and chronic stimulation for continuous stimulation.

[0035] The dry heat drinking water: Dissolve 1 g of saf...

Embodiment 2

[0043] Example 2: Construction of an animal model of abnormal bile carrier UC syndrome

[0044] UC is a chronic inflammatory and ulcerative lesion of the large intestinal mucosa with unknown causes, which has a certain relationship with the incidence of colon cancer and has been listed as one of the modern intractable diseases by the World Health Organization. The current traditional western medicine mainly adopts drug therapy and surgical treatment for UC. Although the drug therapy has a quick effect, it has more side effects after long-term use and recurrence after stopping the drug. The surgical treatment still depends on the surgeon's experience in treating inflammatory bowel disease. Therefore, the research on the mechanism and treatment of UC has become the research focus.

[0045] Uighur medicine believes that the human body is composed of four different body fluids: dry-heat biliary body fluid, damp-heat bloody body fluid, damp-cold mucus-like body fluid, and dry-cold ...

Embodiment 3

[0117] Example 3: Construction of an animal model of abnormal bile carrier UC syndrome

[0118] General morphological analysis of animal models of abnormal bile carrier UC disease: the present invention uses dry heat feed, dry heat environment, tail pinch and noise combined stimulation and 2,4-dinitrochlorobenzene (DNCB) ethanol solution and other means The experimental animals were treated to establish an animal model of abnormal bile carrier UC disease. According to the six indicators of tongue coating, excitement level, skin coat color, stool, urine color and body weight growth index in the modeling process, scores are given in order of severity, and the concentrated scores are selected as the animal model of abnormal bile carrier UC syndrome. standard. These rats with UC syndrome, the carrier of abnormal biliary temperament, all meet the Uighur medicine’s description of the abnormal biliary syndrome population, such as yellow tongue coating, dry; The morphological change...

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Abstract

The invention discloses a method adopting 2,4-dinitrochlorobenzene composite acetic acid method to establish an abnormal bile carrier UC syndrome animal model on the basis of a Uygur medicine body fluid theory. Male wistar line rats are used to establish the animal models, and then subjected to four treatments: dry-hot water feeding, dry-hot feed gavage, dry-hot environment, and mild and continuous stimulation; then a 2,4-dinitrochlorobenzene acetone solution is dropwise added on the backs of the rats, then the rates are forbidden from eating, a pipe is inserted into the colons of the rates from the anus, then a 2,4-dinitrchlorobenzene ethanol solution, an acetic acid solution and normal saline are poured in colon to wash the colon, and finally an abnormal bile carrier UC syndrome animal model is established. The shortage of no related animal model for the research on abnormal bile carrier UC syndrome mechanism and related drug development is overcome, moreover, the modeling method is simple, the modeling rate is high, modeling period is short, the cost is low, the repeatability is good, and the application value is wide.

Description

technical field [0001] The invention relates to the field of medical technology. Specifically, the present invention relates to a technical field that utilizes the Uyghur medicine theory of the Chinese nation and uses scientific methods to practice it. Further, the present invention relates to a method for establishing abnormal bile quality by using 2,4-dinitrochlorobenzene compound acetic acid method. The technical field of carrier UC disease syndrome animal model. Background technique [0002] The construction of animal models is an important technique in medical research. Constructing a disease model similar to human disease can overcome the constraints of long incubation period, many influencing factors, slow development and complex pathogenesis of human disease, better reflect the law of disease occurrence and development, and facilitate the study of different diseases. Diseases that are easy to study in humans themselves. [0003] Ulcerative colitis (Ulcerative Coli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/04A61K31/19
Inventor 库热西·玉努斯王盼闫论张戈
Owner XINJIANG MEDICAL UNIV
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