Method adopting salt-tolerant reinforcement nanometer garbage compost for reinforcing turf grass salt resistance
A technology of nano-garbage composting and lawn grass, which is applied in the field of environmental protection and can solve the problems of no literature reports
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Embodiment 1
[0084] The method for enhancing the salt resistance of turfgrass by using salt-enhanced active nano-garbage composting is characterized in that it is carried out according to the following steps: (1) Preparation of nano-compost:
[0085] Dry the domestic waste compost at 105°C to constant weight, process and grind the waste compost to make 30nm nano-compost for later use;
[0086] (2) Preparation of salt-tolerant microbial bacterial agent:
[0087] Weigh 10g of the compost sample and place it in a sterile Erlenmeyer flask, add 100mL of sterile water and oscillate evenly, then take 10mL of the suspension in a Erlenmeyer flask filled with 100mL of enrichment medium, at 28°C, 220r / min Shaking culture for 3 days is a mixed microbial flora; said mixed microbial flora contains Bacillus subtilis, Bacillus lysinus and Penicillium chrysogenum;
[0088] (3) The salt-tolerance enhancement of mixed microbial flora adopts the method of gradually increasing the salt concentration to reduce...
Embodiment 2
[0102] The method for enhancing the salt resistance of turfgrass by using salt-enhanced active nano-garbage composting is characterized in that it is carried out according to the following steps: (1) Preparation of nano-compost:
[0103] Dry the domestic waste compost at 105°C to constant weight, process and grind the waste compost to make 35nm nano-compost for later use; (2) Preparation of salt-tolerant microbial bacterial agent:
[0104] Weigh 10g of the compost sample and place it in a sterile Erlenmeyer flask, add 100mL of sterile water and oscillate evenly, then take 10mL of the suspension in a Erlenmeyer flask filled with 100mL of enrichment medium, at 28°C, 220r / min Shaking culture for 3 days is a mixed microbial flora; said mixed microbial flora contains Bacillus subtilis, Bacillus lysinus and Penicillium chrysogenum;
[0105] (3) The salt-tolerance enhancement of mixed microbial flora adopts the method of gradually increasing the salt concentration to reduce the impac...
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