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An Efficient Method for Detecting Nephrotoxicity of Nanoparticles

A nanoparticle and nephrotoxicity technology, applied in nanotechnology, biological testing, material inspection products, etc., can solve the problems of difficult to guarantee accuracy, long detection cycle, detection error, etc., to achieve accuracy, low cost, and easy raw materials The effect

Inactive Publication Date: 2017-01-18
武汉光谷新药孵化公共服务平台有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, animal experiments require a long detection period, and detection errors are often caused by individual differences in experimental animals.
In vitro experiments used to replace animal experiments in the prior art have cumbersome steps, are prone to errors due to operational errors, and are difficult to guarantee accuracy

Method used

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  • An Efficient Method for Detecting Nephrotoxicity of Nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Preparation of patterned fiber electrospinning receiving plate: First, coat a positive photoresist on an insulating glass sheet, then cover a layer of photomask, and use a photolithography machine to etch; Deposit a layer of metallic silver on the glass sheet, the shape of the deposited metallic silver is a circular array, and the radius of the circle is 100 μm; finally wash off the remaining positive photoresist;

[0037] 2) Preparation of patterned electrospun fibers: Dissolve medical polymers in organic solvents, use the resultant in step 1) as an electrospinning receiving plate, and prepare patterned electrospun fibers by electrospinning technology; control the diameter of the obtained fibers to 500nm;

[0038] The medical polymer is polylactic acid, and the organic solvent is acetone;

[0039] 3) Place SU-8 on the silicon wafer, remove the rest of SU-8 using photolithography technology, and keep the SU-8 cuboid with a width of 200 μm and a height of 50 μm as a ...

Embodiment 2

[0044] 1) Preparation of patterned fiber electrospinning receiving plate: First, coat a positive photoresist on an insulating glass sheet, then cover a layer of photomask, and use a photolithography machine to etch; Deposit a layer of metallic silver on the glass sheet, the shape of the deposited metallic silver is an elliptical array, the major axis of the ellipse is 200 μm, and the minor axis is 100 μm; finally, the remaining positive photosensitive glue is washed away;

[0045]2) Preparation of patterned electrospun fibers: Dissolve medical polymers in organic solvents, use the resultant in step 1) as an electrospinning receiving plate, and prepare patterned electrospun fibers by electrospinning technology; control the diameter of the obtained fibers to 200nm;

[0046] The medical polymer is polycaprolactone, and the organic solvent is acetone;

[0047] 3) Place SU-8 on the silicon wafer, remove the rest of SU-8 using photolithography technology, and keep the SU-8 cuboid w...

Embodiment 3

[0052] 1) Preparation of patterned fiber electrospinning receiving plate: First, coat a positive photoresist on an insulating glass sheet, then cover a layer of photomask, and use a photolithography machine to etch; A layer of metallic silver is deposited on the glass sheet, and the deposited metallic silver is in the shape of a circular array with a circular radius of 200 μm; finally, the remaining positive photosensitive adhesive is washed away;

[0053] 2) Preparation of patterned electrospun fibers: Dissolve medical polymers in organic solvents, use the resultant in step 1) as an electrospinning receiving plate, and prepare patterned electrospun fibers by electrospinning technology; control the diameter of the obtained fibers to 300nm;

[0054] The medical polymer comprises polylactic acid, the organic solvent is a mixed solution of acetone and dimethylformamide, and the volume ratio of acetone and dimethylformamide is 9:1;

[0055] 3) Place SU-8 on the silicon wafer, rem...

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Abstract

The invention discloses an efficient nanometer particle renal toxicity detection method. The methd comprises the following steps of 1) producing pattern fiber electrospun fiber receiving plates and producing pattern metallic silver layer receiving plates through a photoetching technology and a direct-current magnetron sputtering technology; 2) producing pattern electrospun fibers and performing electrostatic spinning through substances obtained in the step 1); 3) producing PDMS cavities; 4) connecting substances obtained in the step 2) and 3) through plasma treatment; 5) inoculating the original-generation mice renal tubular epithelial cells on the pattern fiber electrospun fibers in the substances obtained in the step 4), pumping media into the cavities obtained in the step 4) through an injection pump, pumping nanometer particles into the cavities, and recording various toxicity indexes within 20 days after the nanometer particles are pumped in. By the aid of the method, efficient and high-accuracy nanometer particle renal toxicity detection can be achieved, detection effects are consistent with in-vivo animal experiments, and the method is quicker than in-vivo animal experiments.

Description

technical field [0001] The invention belongs to the field of nanoparticle toxicity detection, and in particular relates to a method for efficiently detecting nanoparticle nephrotoxicity. [0002] technical background [0003] With the industrialization of nanotechnology, many nanomaterials have been produced on a large scale and applied in coatings, cosmetics, catalysts, new materials, medicine and other fields. People are exposed to the environment with nanomaterials without knowing it. Especially in recent years, the rapid development of nanomaterials in the field of medicine, which is directly related to personal safety and health, has caused the safety of nanomaterials to attract extra attention from all aspects. [0004] The unique physical and chemical properties of nanomaterials determine their unique biological effects. Many studies have shown that nanomaterials may have different toxicity from conventional size substances. [0005] Especially in the field of biomedi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48B82Y35/00
Inventor 刘耀文叶劲松吴贺君陈淑娟何利李美良王淑瑶
Owner 武汉光谷新药孵化公共服务平台有限公司