Trichophyllum 6vs chromosome-specific molecular marker 6vs-bh1 and its use

A 6VS-BH1 and molecular marker technology, applied in the fields of molecular biology and genetic breeding science, can solve the problems of limited EST quantity and molecular markers, and achieve wide annealing temperature, high resolution, and good stability of PCR amplification Effect
CN104877996BInactive Publication Date: 2019-05-31JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2019-05-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a haynaldia villosa's 6VS chromosome specific molecular marker 6VS-BH1 and an application thereof, and belongs to the technical fields of molecular biology and genetic breeding science. The labeled primer 6VS-BH1 is developed on the basis of wheat-brachypodium distachyon comparative genomics means, and the specific sequence is 6VS-BH1F, as shown in SEQ ID NO.1, and 6VS-BH1R, as shown in SEQ ID NO.2. The molecular marker 6VS-BH1 has the advantages of wide annealing temperature range (60-66 DEG C), good stability, strong product brightness, high resolution and the like. The molecular marker 6VS-BH1, as a co-dominant marker, not only can be used for effectively tracing haynaldia villosa's 6VS chromosome in wheat background, but also can be used for distinguishing a homozygote and heterozygote and for distinguishing a Pm21 gene carried translocation line T6VS.6AL and a PmV gene carried translocation line T6VS.6DL. Therefore, the molecular marker 6VS-BH1 disclosed by the invention has an important practical value in the molecular marker-assisted selection breeding of wheat powdery mildew and the pyramiding breeding of Pm21 gene and PmV gene.
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Description

technical field

[0001] The invention relates to a 6VS chromosome-specific molecular marker 6VS-BH1 and its use, belonging to the technical fields of molecular biology and genetic breeding. Background technique

[0002] Wheat powdery mildew is caused by powdery mildew ( Blumeria graminis f.sp. tritici ) is one of the main diseases affecting wheat production. Breeding disease-resistant varieties is the most economical and effective way to avoid the damage of wheat powdery mildew, and the key is the discovery and utilization of powdery mildew resistance genes. As an important wild resource for wheat powdery mildew resistance breeding, wheat tufts ( Dasypyrum villosum ,VV,2 n = 2x = 14) Completely immune or highly resistant to known physiological races of Erysiphe miltiorrhiza, and its disease resistance genes have been successfully transferred to the common wheat background by multiple research groups. For example, Chen Peidu's research group carried the 6VS chromosome ...

Claims

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