Application of Providencia sp. 2D in Remediation of Dibutyl Phthalate Contaminated Soil

A Providencia and contaminated soil technology is applied in the application field of Providencia 2D in the remediation of dibutyl phthalate contaminated soil to achieve the effect of efficient remediation

Active Publication Date: 2018-04-10
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Providencia (Providencia) is a class of bacteria widely present in compost, and there is no report on its degradation of PAEs

Method used

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  • Application of Providencia sp. 2D in Remediation of Dibutyl Phthalate Contaminated Soil
  • Application of Providencia sp. 2D in Remediation of Dibutyl Phthalate Contaminated Soil
  • Application of Providencia sp. 2D in Remediation of Dibutyl Phthalate Contaminated Soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and Identification of Dibutyl Phthalate Degrading Bacteria

[0027] 1. Culture medium formula

[0028] Inorganic salt culture medium (MSM, g / L): K 2 HPO 4 : 5.8; KH 2 PO 4 : 4.5; (NH 4 ) 2 SO 4 : 2.0; MgCl 2 : 0.16; CaCl 2 : 0.02; Na 2 MoO 4 2H 2 O: 0.0024; FeCl 3 : 0.0018; MnCl 2 2H 2 O: 0.0015; adjust the final pH of the inorganic salt culture solution to 7.5. Add DBP so that its concentration in the inorganic salt culture solution is 50 mg / L, and DBP is used as the sole carbon source.

[0029] Beef extract peptone medium (LB): Yeast powder 5.0g, peptone 10.0g, sodium chloride 10.0g, add ultrapure water to 1L, adjust pH=7.0, sterilize at 121°C for 20 minutes. Add 1.5% (W / V) agar powder to the solid plate.

[0030] 2. Separation and identification

[0031]The separation method adopts the sample suspension shaking flask method: Weigh 5g of compost samples (taken from farm animal compost) in a 150mL Erlenmeyer flask containing 50mL o...

Embodiment 2

[0037] Example 2Providencia sp.2D degradation experiment of DBP and its intermediate product phthalic acid

[0038] 1. Preparation of bacterial suspension

[0039] Put the purified strain Providencia sp.2D into 10 mL of LB liquid medium for overnight activation to the logarithmic phase, collect the bacteria by centrifugation at 5000 rpm for 10 min, wash the bacteria with PBS for 3 times and resuspend to adjust the OD 600nm =0.8 as the strain suspension.

[0040] 2. Degradation ability determination

[0041] To the MSM culture fluid (inorganic salt culture fluid g / L) containing different concentrations of DBP (50, 100, 200, 500, 1000mg / L) and phthalic acid (25, 50, 100, 200, 500mg / L) respectively: K 2 HPO 4 : 5.8; KH 2 PO 4 : 4.5; (NH 4 ) 2 SO 4 : 2.0; MgCl 2 : 0.16; CaCl 2 : 0.02; Na 2 MoO 4 2H 2 O: 0.0024; FeCl 3 : 0.0018; MnCl 2 2H 2 O: 0.0015) was inoculated with 1 mL of bacteria, and no bacteria was used as a control, and the pH was adjusted to 8.0, and ea...

Embodiment 3

[0045] Embodiment 3Providencia sp.2D is to the degradation research of DBP in polluted soil

[0046] 1. Preparation of test soil

[0047] The soil is the paddy soil of the farm of South China Agricultural University, the pH is 6.7, the C / N is 5.98, and it is passed through a 60-mesh sieve after air-drying. Animal manure compost was collected from the farm with a pH of 8.8 and a C / N of 17.49. Add compost to the paddy soil according to the common addition ratio on the farm (compost: soil = 1:15, w / w), and mix well to obtain an improved soil with pH = 7.54 and C / N of 10.8. DBP was added to the above-mentioned non-composted soil (conventional soil) and improved soil respectively, so that the concentration reached 100 mg / kg.

[0048] 1. Analysis of DBP degradation effect in soil

[0049] The following treatments were set up in the experiment: conventional soil and improved soil inoculated with bacteria as the experimental group; conventional soil and improved soil without bacter...

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Abstract

The invention belongs to the technical field of biological treatment of environmental pollutants, and specifically discloses the application of Providencia sp. 2D in remediation of dibutyl phthalate-contaminated soil. The bacteria was preserved on January 22, 2015 In China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 2015057; inoculate Providencia sp.2D in DBP-contaminated soil; the DBP degradation rate reached 65% on the 10th day, which was higher than that of the control In addition, in the soil added with compost and inoculated with bacteria, the DBP degradation rate reached 88% on the tenth day, indicating that compost can enhance the degradation of DBP in soil, and the combination of Providenciasp.2D bacteria and compost can effectively repair DBP-contaminated soil has broad application prospects for reducing DBP pollution in agricultural soil environments.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to the application of Providencia sp. 2D in remediation of dibutyl phthalate contaminated soil. Background technique [0002] Dibutylphthalate (di-n-butylphthalate, DBP) belongs to phthalic acid ester compounds (Phthalic acidester, PAEs), is one of the most important plasticizers in the plastics industry, and is also a pesticide, dye detergent It is widely used in food packaging materials, containers, medical equipment and children's toys and other fields. Due to the large number of applications of the above products, DBP has become a global organic pollutant, widely present in the atmosphere, water, soil and organisms. Especially in agricultural production, due to the wide application of plastic films and plastic greenhouses, DBP residues in agricultural soil can easily accumulate in the human body through the food chain. DBP and its metab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 莫测辉赵海明李彦文蔡全英李慧杜欢黄献培鲁磊安
Owner JINAN UNIVERSITY
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