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Microbacterium oxidans and its application in degrading organic pollutants

A technology for oxidizing microbacteria and organic pollutants, applied in the field of biological treatment of environmental pollutants, can solve the problems of maintaining high efficiency, not meeting high-efficiency production, and low degradation rate

Active Publication Date: 2021-06-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1980s, a large number of scholars at home and abroad have screened out some chlorobenzene-degrading microorganisms from sludge, water, and soil, including Psudomonas sp.strain WR1306, P.putida&Bacillus sp., Escherichiahermanii, Pseudomonas sp.strain JS150, Ralstonia sp. .strain JS705, Aeromonas sp., Pseudomonassp.strainFY01&FY04, Bacillussp.StrainFY02&FY03, Pseudononassp.Nov.LP01, etc., the above microorganisms can degrade the pollutant under aerobic or anaerobic conditions, but not under aerobic and anaerobic conditions at the same time Maintain high efficiency, and the degradation rate is low (under aerobic and anaerobic conditions, the degradation rate of p-chlorobenzene in 18d is respectively 50% and 37.2%), which does not meet the requirements of industrial high-efficiency production

Method used

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  • Microbacterium oxidans and its application in degrading organic pollutants
  • Microbacterium oxidans and its application in degrading organic pollutants
  • Microbacterium oxidans and its application in degrading organic pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Isolation, purification, identification and preservation of Microbacterium oxydans ZJUTCB-2

[0019] 1. Screening of strain ZJUTCB-2

[0020] Strain ZJUTCB-2 is a Gram-positive bacterium domesticated and isolated from the activated sludge of Hangzhou Qige Wastewater Treatment Plant. Specific steps are as follows:

[0021]Add 200ml of inorganic salt culture solution to a 340mL serum bottle, pass high-purity nitrogen gas (40mL / min) for 30min to remove the dissolved oxygen in the inorganic salt culture solution and the air at the top of the serum bottle, add 20mL of activated sludge and 987μmol / L Chlorobenzene, at 30°C, 160rpm for enrichment culture. When the concentration of chlorobenzene is 50% of the initial concentration, take out 20mL of the enrichment solution and add it to 200mL of fresh inorganic salt culture solution, then add 987μmol / L chlorobenzene, and cultivate anaerobically at 30°C and 160rpm to the concentration of chlorobenzene 50% of the init...

Embodiment 2

[0033] Example 2: Changes in the concentration of Microbacterium oxydansZJUTCB-2 degrading chlorobenzene in the domestication stage

[0034] Under the optimal environmental factor conditions (culture solution pH=7, culture temperature 30℃), the degradation performance and bacterial growth of the strain ZJUTCB-2 to the initial concentration of 1000μmol / L chlorobenzene were investigated under aerobic conditions. The results showed that the strain could completely degrade 1000μmol / L chlorobenzene, and after the chlorobenzene was completely degraded, the quality of the bacteria ZJUTCB-2 also increased significantly, and the bacterial solution was turbid at this time. The specific implementation steps are as follows:

[0035] Prepare inorganic salt culture solution (same as Example 1), adjust the pH to 7 with sodium hydroxide or dilute hydrochloric acid solution, and distribute it in serum bottles with an average volume of 340 mL, 50 mL per bottle.

[0036] Inoculate the strain ZJ...

Embodiment 3

[0038] Embodiment 3: The degradation of Microbacterium oxydansZJUTCB-2 to different concentrations of chlorobenzene under aerobic conditions

[0039] Under the optimal environmental factor conditions (culture solution pH=7, culture temperature 30℃), the degradation performance and bacterial growth of the strain ZJUTCB-2 to the initial concentration of 700-1500μmol / L chlorobenzene were investigated under aerobic conditions. The results showed that the strain could completely degrade 700-1500 μmol / L of chlorobenzene. After the chlorobenzene was completely degraded, the quality of the bacteria ZJUTCB-2 also increased significantly, and the bacterial solution was turbid at this time. The specific implementation steps are as follows:

[0040] Prepare inorganic salt culture solution (same as Example 1), adjust the pH to 7 with sodium hydroxide or dilute hydrochloric acid solution, and distribute it in serum bottles with an average volume of 340 mL, 50 mL per bottle.

[0041] The pr...

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Abstract

The invention discloses a strain of Microbacterium oxidans and its application in degrading organic pollutants. The application method is: inoculate Microbacterium oxidans into inorganic salt culture solution, add organic pollutants, Under the conditions of 160rpm and pH=6-8, the degradation reaction is carried out to realize the degradation of organic matter; the Microbacterium oxidans of the present invention can degrade chlorobenzene. Microbacterium oxidans can degrade chlorobenzene with an initial concentration of 500‑2000 μmol / L under aerobic conditions, with a removal rate of 100% and an average degradation rate of 334.16549 μmol / (L·h). Under anaerobic conditions, the removal rate of chlorobenzene with an initial concentration of 500‑1500 μmol / L is also 100%. The microbacterium oxidizing agent of the invention can efficiently purify chlorobenzene in industrial waste water and waste gas, has low pollution, is easy to popularize, and has low purification cost.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a strain of Microbacterium oxydans with high-efficiency degradation ability of chlorobenzene and its application in degrading organic pollutants. Background technique [0002] Chlorobenzene is the simplest chlorinated aromatic hydrocarbon. It is insoluble in water, has stable physical and chemical properties, and is difficult to degrade naturally. It is widely used in industrial and agricultural production, basic organic solvents and synthesis of organic compounds. It is also used in medicine, dyes, pesticides and engineering plastics important intermediates in the field. Chlorobenzene can stimulate the skin, conjunctiva and respiratory organs of animals, inhibit the nerve center, and damage the liver and kidneys. Chlorobenzene also has the "three causes" effect. Based on the above hazards, chlorobenzene has been listed as a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34B01D53/84B01D53/68C12R1/01C02F101/36
CPCB01D53/68B01D53/84B01D2257/2064C02F3/34C02F2101/36C12N1/20C12N1/36C12N1/205C12R2001/01Y02A50/20
Inventor 张士汉禹亚男应赞赟叶杰旭陈建孟
Owner ZHEJIANG UNIV OF TECH
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