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Determination method of index components in Chinese medicine raspberry

A determination method, raspberry technology, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve the quality problems of difficult traditional Chinese medicine raspberry, achieve the effect of shortening the total detection time, strong specificity and high stability

Active Publication Date: 2016-12-07
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to scientifically evaluate and control the quality of traditional Chinese medicine raspberry only through the identification of characters and powders.

Method used

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  • Determination method of index components in Chinese medicine raspberry
  • Determination method of index components in Chinese medicine raspberry
  • Determination method of index components in Chinese medicine raspberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Preparation of the test solution

[0063] The Chinese medicine raspberries from 7 origins were treated as follows:

[0064] The Chinese medicine raspberry was crushed, passed through a 40-mesh sieve, accurately weighed 0.5 g, placed in a conical flask, accurately added 50 mL of 70% methanol, tightly plugged, weighed 44.1 g, sonicated at room temperature for 30 min, weighed again, and used 70% methanol. The methanol aqueous solution made up the lost weight, shaken well, centrifuged at 14,000 rpm for 10 min, and took 1 mL of the supernatant.

[0065] Each treatment was repeated 3 times.

[0066] (2) Preparation of reference solution

[0067] Precisely weigh 7.75mg of ellagic acid reference substance, add 100% methanol to dissolve and dilute to 25mL, and prepare 0.31mg·mL -1 Reference stock solution.

[0068] Precisely aspirate 1.5 mL of ellagic acid reference stock solution, and dilute to 5 mL with 100% methanol to prepare 93.00 μg·mL -1 The reference solution wa...

Embodiment 2

[0103] (1) Preparation of the test solution

[0104] Same as step (1) in Example 1.

[0105] (2) Preparation of reference solution

[0106] Precisely weigh 7.75mg and 8.18mg of ellagic acid reference substance and kaempferol-3-O-rutinoside reference substance, respectively, add 100% methanol to dissolve and dilute to 25mL and 10mL respectively, and prepare 0.31mg·mL respectively -1 Ellagic acid reference stock solution and 0.82 mg·mL -1 Kaempferol-3-O-rutinoside reference stock solution.

[0107] Precisely absorb 1.5mL and 0.2mL of ellagic acid reference stock solution and kaempferol-3-O-rutinoside reference stock solution, respectively, and mix, and make up to 5mL with 100% methanol to prepare 93.00μg·mL -1 Ellagic acid, 32.72μg·mL -1 Kaempferol-3-O-rutinoside mixed reference solution was diluted with 1 mL volume fraction of 100% methanol solution, and the concentrations of ellagic acid were 46.50, 23.25, 11.63, 5.81, 2.91, and 1.45 μg·mL, respectively. -1 , the concentr...

Embodiment 3

[0146] (1) Preparation of the test solution

[0147] The Chinese medicine raspberries from 7 origins were treated as follows:

[0148] The Chinese medicine raspberry was crushed, passed through a 40-mesh sieve, accurately weighed 1.0 g, placed in a conical flask, accurately added 50 mL of 70% methanol, tightly plugged, weighed 44.6 g, sonicated at room temperature for 30 min, weighed, and used 70% methanol The aqueous solution made up the lost weight, shaken well, centrifuged at 14,000 rpm for 10 min, and took 1 mL of the supernatant.

[0149] Each treatment was repeated 3 times.

[0150] (2) Preparation of reference solution

[0151] Precisely weighed adenosine, gallic acid, hematoxylin brevis, ethyl gallate, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside and linden glycoside reference substance 5.80mg, 6.90mg, 7.43mg, 6.89mg, 6.33mg, 7.65mg and 5.89mg, respectively, add 100% methanol (containing a small amount of DMSO) to dissolve and dilute to 5mL respectively, accu...

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Abstract

The embodiment of the invention discloses a method for measuring the content of index components in a traditional Chinese medicine, palmleaf raspberry fruit. The method comprises the following steps: (1) preparing a test sample solution; (2) preparing a mixed contrast solution; (3) performing ultra high performance liquid chromatography detection; (4) constructing standard curves of N index components; and (5) determining the content of each of the N index components in a test sample. The method for measuring the content of the index components in the traditional Chinese medicine, palmleaf raspberry fruit, is accurate and efficient and can be used for controlling the quality of the traditional Chinese medicine, palmleaf raspberry fruit; furthermore, the method can be used for simultaneously detecting the various index components in the traditional Chinese medicine, palmleaf raspberry fruit; compared with an existing method for respectively detecting different index components, the method disclosed by the invention has the advantages that the total detection time is shortened, and the reagent cost is reduced; meanwhile, the detection result is accurate, the precision is high, the repetitiveness is high, the stability is high, and the specificity is excellent.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicines, in particular to a method for measuring the content of index components in traditional Chinese medicine raspberries. Background technique [0002] Chinese medicine raspberry is the dried fruit of Rubus chingii Hu, a plant of the Rosaceae family. In early summer, the fruit is harvested when the green turns green and yellow, the stems and leaves are removed, slightly scalded or steamed in boiling water, taken out, and dried to obtain the traditional Chinese medicine raspberry. The traditional Chinese medicine raspberry has the effect of invigorating the kidney, consolidating the essence and reducing the urine, nourishing the liver and improving the eyesight. Modern pharmacological research shows that raspberry has antioxidant, anti-inflammatory, antithrombotic, anticancer and other activities, and its chemical components mainly include flavonoids, terpenes, steroids,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 柴欣杨静杜龙飞江振作李洁朱彦王跃飞贺英
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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