Cultchless oyster intermediate culture method based on ecological system
A single oyster, intermediate cultivation technology, applied in the field of aquaculture, can solve the problems of oyster seed death, unable to meet the growth needs of oyster seedlings, and high cost, and achieve the effects of sustainable utilization, high-density cultivation and easy management.
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[0031] 1. Baby choice
[0032] Fujian oysters of 2-3 years old, body weight ≥ 40g, shell length ≥ 10cm, robust, well-developed gonads, which can be directly induced to give birth or cultivated through short-term artificial enhancement are selected as broodstock.
[0033] 2. Insemination and triploid induction
[0034] Scrape the ovary with a scalpel and put it in a container, stir it up; filter it with a 200-mesh sieve to make it a cell suspension, and wash it 3 times. Pick male shellfish according to 10% of the number of female shellfish, and use the same method to obtain semen; add it to seawater with eggs, and stir for 5 minutes. Then use clean sea water to repeatedly wash away excess semen and impurities.
[0035] Triploid oysters were obtained by cytochalasin B treatment, and ploidy detection was performed by flow cytometry. The ploidy of the triploid seed needs to pass the ploidy check to see if the triploid commercial seed is successfully obtained.
[0036] 3. Larva...
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