A kind of anti-tumor polypeptide nano drug and its preparation method and application
A nano-drug and anti-tumor technology, applied in the direction of anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as insufficient stability and molecular structure damage, and achieve the effects of improving bioavailability, prolonging half-life, and good therapeutic effect
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Embodiment 1
[0073] In this example, the anti-tumor polypeptide nano drug was prepared by the following method, which is:
[0074] M. Lourdes Ponce et al., Cancer Research, 2003, 63: 5060-5064 provide the C16Y polypeptide that inhibits tumor angiogenesis and tumor growth (the sequence starting from the amino terminal is: aspartic acid-phenylalanine-lysine amino acid-leucine-phenylalanine-alanine-valine-tyrosine-isoleucine-lysine-tyrosine-arginine) as a hydrophilic anti-tumor polypeptide, according to Literature (Lihong Liu et al., Nature Nanotechnology, 2009, 4:457-463 and Ying Zhao et al., J Control Release, 2014, 177:11-19) provide solid-phase synthesis and peptide purification methods, the eight leucine The hydrophobic polypeptide composed of 2 glycines is connected to the anti-tumor polypeptide C16Y, and the tripeptide formed by 3 lysines is connected at the amino terminal of the hydrophobic polypeptide, and then the amino group on the lysine (including the terminal amino group and si...
Embodiment 2
[0087] In this example, through the same synthesis method and steps as in Example 1, 3 isoleucine, 2 methionine and 1 lysine were coupled to the anti-tumor polypeptide C16Y, and the lysine DEAP molecules are connected to the terminal amino group and the side chain amino group, and the amphipathic anti-tumor polypeptide coupled with DEAP is obtained. The anti-tumor polypeptide nano drug system was obtained through the same self-assembly process as in Example 1.
[0088] The structure of the amphiphilic anti-tumor polypeptide coupled with DEAP obtained in this example is confirmed by means of high performance liquid chromatography and mass spectrometry: C16Y-(tripeptide formed by isoleucine)-(dipeptide formed by methionine) )-lysine-(DEAP) 2 .
[0089] The morphology and particle size of the obtained anti-tumor polypeptide nano-drug system were characterized by transmission electron microscope and laser particle size analyzer. It is 40-200nm, the average particle size is abou...
Embodiment 3
[0091] In this example, through the same synthesis method and steps as in Example 1, 20 alanines and 5 lysines were coupled to the anti-tumor polypeptide C16Y, and the terminal amino group and side of lysine A DEAP molecule is connected to the chain amino group, and an amphipathic anti-tumor polypeptide coupled with DEAP is obtained. The anti-tumor polypeptide nano drug system was obtained through the same self-assembly process as in Example 1.
[0092] The structure of the amphiphilic anti-tumor polypeptide coupled with DEAP obtained in this example was confirmed by means of high performance liquid chromatography and mass spectrometry: C16Y-(polypeptide formed by 20 alanines)-(5 lysines Polypeptides formed)-(DEAP) 6 .
[0093] The morphology and particle size of the obtained anti-tumor polypeptide nano-drug system were characterized by transmission electron microscope and laser particle size analyzer. It is 30-160nm, the average particle size is about 100.5nm, and the disp...
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