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Magnetic biological material capable of adsorbing uranium (VI) in aqueous solution and preparation method of magnetic biological material

A technology of biomaterials and aqueous solutions, applied in the field of environmental biotechnology applications

Inactive Publication Date: 2015-09-30
EAST CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] So far, there have been no relevant reports at home and abroad on whether expressing MTs on the outer membrane of S.

Method used

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  • Magnetic biological material capable of adsorbing uranium (VI) in aqueous solution and preparation method of magnetic biological material
  • Magnetic biological material capable of adsorbing uranium (VI) in aqueous solution and preparation method of magnetic biological material
  • Magnetic biological material capable of adsorbing uranium (VI) in aqueous solution and preparation method of magnetic biological material

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Embodiment Construction

[0039] 1. Preparation of ferrofluid

[0040] Weigh FeCl 3 19.4652g (0.12mol), FeSO 4 20.016g (0.072mol) of 7H2O, mixed with 200ml of distilled aqueous solution, N 2 Under protection (first pass into N 2 , in the form of air bubbles) heated to 85°C-90°C, stirred at high speed, added dropwise excess ammonia water, aged for 30min, and cooled. Separation by magnetic precipitation, fully washing with deionized water, suction filtration, washing with ethanol (drying quickly), and vacuum freeze-drying.

[0041] 2. Preparation of magnetic biomaterials

[0042] Dissolve 0.1g of freeze-dried recombinant Saccharomyces cerevisiae cells and 0.01g of dried ferrofluid in 10ml of distilled water, briefly ultrasonically disperse and mix well, then mix with 40ml of 1% sodium alginate, mix well, and spray The magnetic microspheres were prepared by immobilizing in 5% calcium chloride solution for 30 minutes.

[0043] The magnetic microspheres are collected by a magnet, washed 2 to 3 times wit...

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Abstract

The invention discloses a magnetic biological material capable of adsorbing uranium (VI) in an aqueous solution and a preparation method of the magnetic biological material. The magnetic biological material has the particular name of gene recombinant saccharomyces cerevisiae microsphere, which is obtained by mixing human liver metallothionein gene recombinant saccharomyces cerevisiae with a ferromagnetic fluid and immobilizing by sodium alginate and calcium chloride; wherein the mixing ratio is that the mass ratio of gene recombinant saccharomyces cerevisiae bacteria to the ferromagnetic fluid is 10:1. The magnetic gene recombinant saccharomyces cerevisiae microsphere has such characteristics of gene recombinant saccharomyces cerevisiae as high uranium (VI) adsorption capacity, low adsorption cost, recyclable biomass and no secondary pollution, and is relatively convenient and rapid to recycle, thereby greatly enhancing the recycling ratio, reducing the uranium pollution degree, and meanwhile promoting the uranium resource recycling actively.

Description

technical field [0001] The invention relates to a magnetic biological material for absorbing uranium (VI) in aqueous solution and a preparation method thereof, belonging to the field of environmental biotechnology application. Background technique [0002] Biosorption refers to the process of making heavy metal ions adsorbed by biological cells through a series of biochemical actions. Studies have shown that a variety of microorganisms are used for the biosorption of heavy metal ions, including algae, bacteria, fungi, and yeast. Among them, Saccharomyces cerevisiae has been shown to be effective on Cu 2+ 、Cr 3+ , Pb 2+ , Hg 2+ 、Cd 2+ 、As 3+ And many other metal ions have strong adsorption capacity, and at the same time have the excellent characteristics of safety, easy cultivation and growth. Microbial adsorption has low cost, high efficiency, reusable biomass, and no secondary pollution. It has broad application prospects in treating uranium-containing wastewater and...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30C02F1/28C02F1/62
Inventor 李敏梁波王金扣马腾腾张志宾刘云海于梁梁
Owner EAST CHINA UNIV OF TECH
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