Microwave-assisted aqueous two-phase extraction process of Farfugium japonicum general flavone and application of Farfugium japonicum general flavone

A microwave-assisted, total flavonoid technology is applied in the directions of medical preparations containing active ingredients, plant raw materials, antibacterial drugs, etc., which can solve the problems of difficulty in obtaining pure Dawufeng total flavonoids, limitations, etc., and achieves low cost and high efficiency. Distribution coefficient, easy-to-operate effect

Inactive Publication Date: 2015-11-11
HANDAN COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are very few data on the extraction method and antibacterial activit...

Method used

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  • Microwave-assisted aqueous two-phase extraction process of Farfugium japonicum general flavone and application of Farfugium japonicum general flavone
  • Microwave-assisted aqueous two-phase extraction process of Farfugium japonicum general flavone and application of Farfugium japonicum general flavone
  • Microwave-assisted aqueous two-phase extraction process of Farfugium japonicum general flavone and application of Farfugium japonicum general flavone

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Embodiment 1

[0027] A microwave-assisted aqueous two-phase extraction process for total flavonoids of Dawufeng, comprising the following steps:

[0028] 1) Extraction of Dawufeng Crude Extract

[0029] Accurately weigh 0.5Kg of Dawufeng powder, add 15L of ethanol solution with a volume fraction of 60% extractant, mix with Dawufeng powder, and extract. First, microwave the mixture at 25°C for 25 minutes, then place it in a constant temperature water bath at 60°C for 1.5 hours, centrifuge, and take the supernatant to obtain the crude extract;

[0030] 2) Aqueous two-phase extraction

[0031] The crude extract obtained in step 1) was added to C 2 h 5 OH-(NH 4 ) 2 SO 4 The aqueous two-phase system that forms makes the mass fraction of the crude extract in the aqueous two-phase system be 19%, and the C 2 h 5 The mass fraction of OH is 20%, the (NH 4 ) 2 SO 4 The mass fraction is 16%, adding NaCl with a mass fraction of 2.68%, adding hydroxypropyl-β-cyclodextrin with a mass fraction o...

Embodiment 2

[0035] A microwave-assisted aqueous two-phase extraction process for total flavonoids of Dawufeng, comprising the following steps:

[0036] 1) Extraction of Dawufeng Crude Extract

[0037] Accurately weigh 0.5Kg of Dawufeng powder, add 15L of ethanol solution with a volume fraction of 60% extractant, mix with Dawufeng powder, and extract. First, microwave the mixture at 25°C for 20 minutes, then place it in a constant temperature water bath at 60°C for 1 hour, centrifuge, and take the supernatant to obtain a crude extract.

[0038] 2) Aqueous two-phase extraction

[0039] The crude extract obtained in step 1) was added to C 2 h 5 OH-(NH 4 ) 2 SO 4 The aqueous two-phase system that forms makes the mass fraction of the crude extract in the aqueous two-phase system be 19%, and the C 2 h 5 The mass fraction of OH is 28%, the (NH 4 ) 2 SO 4 The mass fraction is 24%, NaCl with a mass fraction of 1.5% is added, and hydroxypropyl-β-cyclodextrin with a mass fraction of 0.3% ...

Embodiment 3

[0043] A microwave-assisted aqueous two-phase extraction process for total flavonoids of Dawufeng, comprising the following steps:

[0044] 1) Extraction of Dawufeng Crude Extract

[0045] Accurately weigh 0.5Kg of Dawufeng powder, add 15L of ethanol solution with a volume fraction of 60% extractant, mix with Dawufeng powder, and extract. First, microwave the mixture at 25°C for 20 minutes, then place it in a constant temperature water bath at 60°C for 1 hour, centrifuge, and take the supernatant to obtain a crude extract.

[0046] 2) Aqueous two-phase extraction

[0047] The crude extract obtained in step 1) was added to C 2 h 5 OH-(NH 4 ) 2 SO 4 The aqueous two-phase system that forms makes the massfraction of the crude extract in the two-phase system be 10%, and the C 2 h 5 The mass fraction of OH is 24%, the (NH 4 ) 2 SO 4 The mass fraction is 18%, add 3% NaCl, add 0.4% hydroxypropyl β-cyclodextrin, adjust the pH value to 8, mix, and separate phases. The phase sep...

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Abstract

The invention relates to a microwave-assisted aqueous two-phase extraction process of Farfugium japonicum general flavone. The process includes the following steps: obtaining crude extract through microwave-assisted extraction of Farfugium japonicum; and extracting the crude extract through an aqueous two-phase system formed by C2H5OH-(NH4)2SO4 to obtain Farfugium japonicum general flavone. By the process, the extraction rate of crowndaisy chrysanthemum general flavone is increased, the extraction rate of impurity ingredients is decreased, and purity and yield of Farfugium japonicum general flavone which is obtained finally are improved remarkably.

Description

technical field [0001] The invention relates to an extraction process of plant effective components, in particular to a microwave-assisted two-phase extraction process and a response surface optimization method for total flavonoids of Dawufeng. Background technique [0002] Farfugium japonicum (L.f.) Kitam. is a perennial herb, the whole plant is used as medicine. Alias ​​lotus grass ("Fujian folk herbal medicine"), unipedal lotus ("Interrogation of Materia Medica"), wild pumpkin ("Zhejiang folk herbal medicine"). The young leaves and stems of Dawufengcao can be eaten as vegetables, "pungent, warm, non-toxic, cool in nature, bitter in taste", and have the effects of reducing fever, detoxifying, and promoting blood circulation. Folks are widely used to treat eczema, cough, bronchitis, lymphadenitis, dysentery and other diseases. Dawufengcao has a high development value as a resource plant for both medicine and food. Flavonoids have various physiologically active medicinal ...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P31/04
Inventor 陈建中葛水莲
Owner HANDAN COLLEGE
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