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In-vitro conservation method for liparis nervosa

A technology of in vitro preservation and see blood green, which is applied in the field of in vitro preservation of plants see blood green, to achieve the effect of alleviating over-harvesting

Active Publication Date: 2015-12-02
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the method of in vitro preservation of blood blue in the prior art.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Materials: See Liparisnervosa (Thunb. ex A. Murray) Lindl.).

[0018] 2. Material category: see Xueqing seeds.

[0019]3. Culture conditions: (1) Seed germination medium: Basic medium is 1 / 2MS (macronutrients in MS are halved, and the rest are the same as MS); (2) Bud proliferation medium: MS+B5 vitamins+IAA0. 5mg / L+KT0.5mg / L+inositol 100mg / L; (3) in vitro preservation medium: 1 / 2MS+PPP 333 2.0mg / L+NAA0.5mg / L. Above medium (1) and (2) add 20g / L sucrose, (3) add 40g / L sucrose, activated carbon 1-2g / L. All media were solidified by adding agar 5.8g / L, pH5.8, culture temperature was 25±2℃, light intensity was 10-20μmol / (m 2 s), light 12h / d. The culture bottles used are all white transparent glass bottles, with a diameter of 7.5cm x height of 9.0cm, and a volume of about 300ml; the medium used in (1) and (2) is about 40ml, and the medium used in (3) for in vitro preservation is about 75ml .

[0020] 4. Growth and differentiation

[0021] 4.1 Obtaining of explants: ...

Embodiment 2

[0026] 1. Materials: See Liparisnervosa (Thunb. ex A. Murray) Lindl.).

[0027] 2. Material category: see Xueqing seeds.

[0028] 3. Culture conditions: (1) Seed germination medium: Basic medium is 1 / 2MS (macronutrients in MS are halved, and the rest are the same as MS); (2) Bud proliferation medium: MS+B5 vitamins+IAA0. 5mg / L+KT0.5mg / L+inositol 100mg / L; (3) in vitro preservation medium: 1 / 2MS+PPP 333 2.0mg / L+NAA0.5mg / L. Above medium (1) and (2) add 20g / L sucrose, (3) add 40g / L sucrose, activated carbon 1.5g / L. All media were solidified by adding 5.8g / L of agar, pH5.8, the culture temperature was 25±2℃, and the light intensity was 15μmol / (m 2 s), light 12h / d. The culture bottles used are all white transparent glass bottles, with a diameter of 7.5cm x height of 9.0cm, and a volume of about 300ml; the medium used in (1) and (2) is about 40ml, and the medium used in (3) for in vitro preservation is about 75ml .

[0029] 4. Growth and differentiation

[0030] 4.1 Obtaining ...

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Abstract

The invention provides an in-vitro conservation method for liparis nervosa which has an extremely high medicinal value and ornamental potential. According to the method, through the steps of liparis nervosa seed germination, clustered bud induction, in-vitro conservation and the like, to be specific, fruit pods of liparis nervosa are subjected to surface sterilization, then the fruit pods are broken so as to take out explant seeds, the explant seeds are inoculated on a seed germination culture medium, seedlings with leaves grow and are cultured on a clustered bud proliferation culture medium, so that pseudobulb is obtained, the pseudobulb is inoculated on an in-vitro conservation culture medium, and seedlings subjected to in-vitro conservation are transplanted after being subjected to conventional seedling hardening, therefore, a rapid propagation and in-vitro conservation system of liparis nervosa is built and the subculture cycle is 3 years or above. The method provided by the invention provides an important theory source and technical support for development, utilization and protection of liparis plant germplasm resources.

Description

Technical field: [0001] The invention relates to the field of plant biotechnology, in particular to a method for preserving Liparisnervosa (Thunb. Lindl.) in vitro. Background technique: [0002] Liparisnervosa (Thunb.Lindl.) is a ground herb of the Orchidaceae (Orchidaceae) genus Liparis, widely distributed in tropical and subtropical regions of the world. Its stem (or pseudobulb) is cylindrical and fleshy. It is a commonly used traditional Chinese medicine in the folk. It has the effects of clearing heat and detoxifying, cooling blood and stopping bleeding. It can be used for various bleeding diseases, snake bites, dermatitis, and traumatic injuries. The polysaccharide extract and total alkaloids of Jianxueqing have good antioxidant and antibacterial effects, and have high medicinal value; at the same time, Jianxueqing also has ornamental value and is a good material for potted flowers. See Xueqing has been listed as a national second-class protected plant. At present, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何俊孟静李春芳杨俊波王红李德铢
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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