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Immunoglobulin E immunoturbidimetry detection kit

An immunoglobulin and detection kit technology, applied in the field of immunoglobulin E immunoturbidimetric detection kits, can solve the problems of low accuracy and sensitivity, limited popularization and application, poor stability of detection reagents, etc. Enhanced stability and improved analytical sensitivity

Inactive Publication Date: 2015-12-09
章丘维他力医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ordinary immunoglobulin E immunoturbidimetric assay reagents are not stable, and the accuracy and sensitivity are not high, which limits its clinical application.

Method used

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  • Immunoglobulin E immunoturbidimetry detection kit
  • Immunoglobulin E immunoturbidimetry detection kit
  • Immunoglobulin E immunoturbidimetry detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A conventional detection kit for immunoglobulin E immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0025] Wherein the reagent R1 consists of:

[0026] pH7.6Tris buffer 18.16mmol / L

[0027] Sodium chloride 150mmol / L

[0028] Macrogol 4006%

[0029] Reagent R2 consists of:

[0030] Tris buffer pH7.618.16mmol / L

[0031] Goat anti-human IgE antibody 30ml / L

[0032] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0033] Add 5 μl of physiological saline, sample or calibrator, then add 200 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 100 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0034] The calibrator used in this example is the IgE calibrator produced by Jiufeng Runda Biotechnology Co., Ltd.

Embodiment 2

[0036] An immunoglobulin E immunoturbidimetric detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0037] Wherein the reagent R1 consists of:

[0038]

[0039] Reagent R2 consists of:

[0040]

[0041]Concrete determination method is with embodiment 1.

Embodiment 3

[0043] An immunoglobulin E immunoturbidimetric detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0044] Wherein the reagent R1 consists of:

[0045]

[0046] Reagent R2 consists of:

[0047]

[0048]

[0049] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses an immunoglobulin E immunoturbidimetry detection kit, and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2, and a calibration material. By adding a certain amount of silica coated magnetic nanoparticles into the reagent R1 and adding a certain amount of bovine serum albumin and Kathon-CG into the reagent R2, the stability and the analysis sensitivity of the kit are effectively improved, the linear range is also better, the reagent accuracy is high, and further popularization and application in markets are facilitated.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an immunoglobulin E immunoturbidimetric detection kit. Background technique [0002] There are five classes of immunoglobulins, namely Immunoglobulin G (IgG), Immunoglobulin A (IgA), Immunoglobulin M (IgM), Immunoglobulin D (IgD) and Immunoglobulin E. Its main function is to have an immune reaction with the antigen to generate an antigen-antibody complex, thereby blocking the harm of the pathogen to the body and making the pathogen lose its pathogenic effect. On the other hand, immunoglobulins sometimes have pathogenic effects. Clinical allergic symptoms such as bronchospasm caused by pollen, systemic allergic reaction caused by penicillin, skin urticaria (commonly known as wheal rash) and so on. Immunoglobulin preparations can enhance the anti-virus ability of the human body and can be used as medicine. For example, injecting human serum or gamma ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54326
Inventor 甘宜梧董雯谭柏清赵新
Owner 章丘维他力医疗器械有限公司
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