Image super-resolution reconstruction method for FPM algorithm based on nonlinear optimization
A technology of super-resolution reconstruction and nonlinear optimization, applied in image enhancement, image data processing, calculation, etc., can solve the problems of small magnification of the observed object and narrowed field of view, and achieve the effect of improving the reconstruction effect.
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[0021] Below in conjunction with embodiment the present invention is further described.
[0022] The FPM platform used in the following examples includes a microscope, the microscope is provided with a camera (i.e. an imaging device), and the light source of the microscope adopts a programmable LED array. The distance between the LED array and the stage is generally 6-12cm, and 8cm is selected in this example. The FPM platform is mainly based on the microscope. Compared with the traditional microscope, a programmable LED array is placed at the light source to replace the original light source.
[0023] In some embodiments, the image super-resolution reconstruction method based on the FPM algorithm of nonlinear optimization comprises the following steps:
[0024] (1) Collect low-resolution images I under different angles of illumination with the FPM platform i (x,y):
[0025] Using a computer to control the light emission of the LED array and the image acquisition of the cam...
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