Soil cultivation method for potato virus-free basic seeds

A cultivation method and original seed technology, applied in the field of potato virus-free seedling cultivation, can solve the problems of high cost of virus-free seedling cultivation and long demand time, and achieve the effects of reducing cultivation cost, market price and pollution rate

Active Publication Date: 2015-12-16
定西金麟种业有限责任公司
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Problems solved by technology

[0004] The purpose of the present invention is to: overcome the problems of high cost and long time required for the cultivation of virus-free seedlings in the prior art, thereby providing a soil cultivation method for the original seed of virus-free potato, which has the advantages of low cost and short cultivation time

Method used

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Embodiment 1

[0021] Potato detoxification original original seed soil culture method, comprises the following steps:

[0022] 1) Production of tissue culture seedlings: adopting improved MS medium to cultivate detoxified tissue culture plantlets, which reduces the pollution rate of tissue culture production, wherein the improved MS medium is added 50% carbendazim in MS medium Wettable powder 5mg / L.

[0023] 2) Rooting and strong seedlings: Take out the tissue culture seedlings suitable for colonization from the culture bottle, remove the root system and wash the medium, soak the roots with NAA50mg / L+2-4D5mg / L rooting agent for 10-15 minutes, and then colonize In the soilless culture substrate, among them, the soilless cultivation substrate uses vermiculite and organic fertilizer, mixed with carbendazim and myclostrobin for substrate antibacterial, and at the same time, uses plastic bow shed for heat preservation and moisture retention, so as to promote the rooting and rapid growth of tissu...

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Abstract

The invention discloses a soil cultivation method for potato virus-free basic seeds. The method comprises: 1) production of a tissue culture seedling; 2) rooting and strengthening; 3) soil cultivation; and 4) harvesting and storage, wherein the step 2) rooting and strengthening comprise: taking out the tissue culture seedling suitable for fixed planting out of a culture bottle; removing root systems and cleaning a culture medium; soaking the roots for 10-15 minutes by using a NAA50 mg/L+2-4 D 5 mg/L rooting agent; then fixedly planting the roots in a soilless culture medium, and keeping the temperature and humidity by using a plastic arch-shaped shed to accelerate the tissue culture seedling to take root, sprout and quickly grow; 12 days after fixed planting, starting spraying a nutritional liquid, spraying the nutritional liquid once at an interval of five days, and spraying the nutritional liquid three times; in 29-32 days after fixed planting, uncovering plastic of the arch-shaped shed, and selecting robust tissue culture seedlings, wherein the height of the seedlings reaches 10 cm and the blades are longer than 1 cm, and transplanting the tissue culture seedlings in pre-treated field soil. By adopting the soil which replaces the medium to culture the potato virus-free basic seeds, the production cost is greatly lowered, so that the market supply price is lowered, and the method plays a positive role in generalizing and popularizing virus-free potatoes.

Description

technical field [0001] The invention belongs to the technical field of potato virus-free seedling cultivation, and in particular relates to a method for soil cultivation of potato virus-free original seeds. Background technique [0002] Potato has a long history of planting in my country and is the fourth largest cultivated crop after wheat, rice and corn. It has high nutritional value, huge potential for increasing production and broad prospects for industrial development. In recent years, due to the aggravation of environmental pollution, the probability of virus infection in potatoes has increased during planting. Potatoes are asexually propagated crops, and the viruses infected by them can accumulate from generation to generation, causing serious degradation of seed potatoes and seriously affecting the development of the potato industry. . With the deepening of potato research, it was found that although the virus-infected potato plants contained a large amount of virus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00A01H4/00
CPCA01G31/00A01H4/001A01H4/005A01H4/008Y02P60/21
Inventor 袁安明张明徐祺昕张小静陈自雄谭伟军
Owner 定西金麟种业有限责任公司
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