The invention discloses a method for producing short-day strawberry flowering seedlings in the central region of Yunnan province. The method comprises the steps of variety selecting, stock plant field planting curing, seedling breeding and short-day flowering processing. The short-day strawberry variety with the properties that the number of flowers in simple inflorescence is more than 20, the flowering time of a single flower in the same inflorescence is scattered, and the flowering time of the simple inflorescence lasts for one or more months is selected; the stock plant field planting curing, the seedling breeding and short-day flowering processing are carried out in the central region of Yunnan province with the altitude ranging from 1,600 meters to 1,900 meters; a nutrient solution including 100-150 milligrams per liter of N, 20-25 milligrams per liter of P and 80-100 milligrams per liter of K is used for watering the seedlings during the stock plant curing period, the seedlings are processed for 10 hours in the short day and processed for 30-35 days under the natural temperature condition, and flower bud differentiation is achieved in more than 90 percent of seedlings. The flowering cost is low, the flowering seedling supply time is early, 30-40 seedlings can be harvested in each stock plant in the middle ten days of June, the fresh autumn short-day strawberries can be produced, and normal winter and spring fresh strawberry production can be guaranteed.