Three-channel microscope interface

Abstract

The invention discloses a three-channel microscope interface which includes a housing, a beam splitting module and a tube lens. Three light holes are disposed in the housing. The light splitting module is disposed in the housing, and includes a first dichroscope and a second dichroscope which are arranged in parallel. Incident mixed light passes through the tube lens and falls onto the first dichroscope to produce first transmitting light and first reflecting light. The first transmitting light emits from the light holes at the corresponding position on the housing. The first reflecting light falls onto the second dichroscope to produce second transmitting light and second reflecting light. The second transmitting light and the second reflecting light emit respectively through the light holes at the corresponding positions on the housing. Optical paths of the incident light from the tube lens to image planes of the light holes are the same. The interface allows three cameras to share one imaging tube lens, the imaging amplification factors of the channels are exactly the same, and when large samples are subjected to scan imaging, no problem will occur in the stitching of the channels and the matching of all the channels.

Description

technical field [0001] The invention belongs to the field of optical imaging, and more specifically relates to a three-channel microscope interface. Background technique [0002] Photomicrography is an integral part of the use of modern microscopes. Due to the continuous development of fluorescence imaging, in the field of multi-spectral color separation imaging that pursues speed, such as the acquisition of multi-color microscopic images of large samples or the acquisition of multi-color microscopic images of a large number of samples, simultaneous three-band imaging is required. [0003] But at present, there is no microscope interface to achieve three-channel imaging, especially for scientific research cameras, which are relatively large in size, making it difficult for them to share one imaging tube lens. If we use a relay lens, not only the structure is complicated, but also the shooting area and image magnification of the three cameras are difficult to adjust, which w...

AI Technical Summary

Problems solved by technology

[0003] But at present, there is no microscope interface to achieve three-channel imaging, especially for scientific research cameras, the size is large, and it is difficult for them to share one imaging tube lens

If we use a relay lens, not only the structure is complicated, but also the shooting area and image magnification of the three cameras are difficult to adjust, which will bring great problems to the image matching, especially when large samples are scanned and stitched. obvious

Images