Traditional Chinese medicine composition used for bacterial diseases of turtles

A Chinese herbal medicine composition and bacterial disease technology, which is applied in the field of traditional Chinese medicine composition for soft-shelled turtle breeding, can solve the problems of lack of in-depth research on soft-shelled turtle bacterial diseases, and achieve the effects of less toxic and side effects, prevention of bacterial diseases, and improvement of body weight and meat quality

Inactive Publication Date: 2015-12-23
长沙县明利水产养殖有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is a lack of in-depth research on the treatment of Chinese herbal medicines for soft-shelled turtle bacterial diseases, and it is urgent to develop Chinese herbal medicine compositions with definite curative effects and controllable costs

Method used

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  • Traditional Chinese medicine composition used for bacterial diseases of turtles

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Comparison scheme
Effect test

Embodiment 1

[0013] The isolation and identification of embodiment 1 pathogenic bacteria

[0014] According to literature reports, there are three main pathogenic bacteria of turtle bacteriosis: Aeromonas sobria, Aeromonas hydrophila and Aeromonas caviae. Dying Chinese soft-shelled turtles suffering from typical symptoms of bacterial diseases such as red neck disease, perforation disease, rotting skin disease, red floor disease, and white floor disease were selected from several turtle farms in Yueyang and Changde, Hunan, as test materials. A total of 36 strains of the above three bacteria were identified, and 3 strains of bacteria were randomly selected for cultivation for further experiments.

Embodiment 2

[0015] The preparation of embodiment 2 Chinese medicine composition

[0016] Eucommia leaf water extract: provided by Hengxing Biological Company, Zhangjiajie City, Hunan Province. (The content of chlorogenic acid is up to 8%, and it is obtained by extracting Eucommia leaves as raw materials and using water as a solvent.

[0017] Jiuliguang water extract: take the dried stems and leaves (10kg) of the herbal medicine Jiuliguang plant as raw materials, pulverize them with a pulverizer, pass through a 60-mesh sieve, add 25Kg of distilled water and soak at room temperature for 30 minutes, add steam to heat to boiling, maintain boiling and soak for 40 minutes minutes, filtered, concentrated, and spray-dried to obtain 952 grams of light yellow powder.

[0018] The water extract of Jiuliguang and the water extract of Eucommia leaves are fed according to the weight ratio of 5:2, mixed by a small powder mixer, and the traditional Chinese medicine composition of the present invention i...

Embodiment 3

[0019] Embodiment 3 in vitro antibacterial test

[0020] Three strains of Aeromonas sobria, Aeromonas hydrophila and Aeromonas caviae were tested, all of which were isolated from the diseased soft-shelled turtle. Inoculate the strain in the broth culture solution, culture at 28°C for 24 hours, culture on a plate, and then dilute with sterile saline to contain 10 bacteria 6 CFU / ml, put in the refrigerator for later use.

[0021] The test drug is now dissolved in appropriate amount of distilled water to dissolve the composition of ① Eucommia leaf water extract ② Jiuliguang water extract ③ Jiuliguang water extract: Eucommia ulmoides leaf water extract = 5: 2, and then use broth culture solution respectively Proportionally diluted to prepare culture solutions containing extracts with different concentrations.

[0022] For the antibacterial test, a 40-well tissue culture plate was used, and 190 μl of culture solution with different drug concentrations and 10 μl of bacterial solut...

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Abstract

A composition which consists of traditional Chinese herbal medicines Artemisia anomala water extract and folium cortex eucommiae water extract in weight ratio of (2 to 3):1 is adopted by the invention to treat bacterial diseases of turtles, such as red neck disease, shothole disease, necrotic skin disease, red abdominal shell disease and white abdominal shell disease. The composition has an exact curative effect, is capable of controlling the bacterial diseases of the turtles at low cost, has small toxic and side effects and no drug residues, and can be used for preventing the bacterial diseases for a long term; discovered after long-term use, the composition disclosed by the invention is also capable of obviously improving the weight and the meat quality of artificial bred turtles.

Description

technical field [0001] The invention relates to a Chinese medicine composition for aquaculture, in particular to a Chinese medicine composition for turtle breeding. Background technique [0002] Soft-shelled turtles are commonly known as soft-shelled turtles, water fish, turtles and tortoises, etc. They are oviparous reptiles and live amphibiously on land and water. Soft-shelled turtle meat is delicious and nutritious. It has the effects of clearing heat and nourishing yin, calming the liver and quenching wind, softening and resolving hard masses. Because soft-shelled turtle has great edible and medicinal value, artificial breeding has been widely carried out all over the country. Due to the high degree of intensive artificial breeding and high breeding density, it is easy to breed germs and cause diseases, such as bacterial diseases, viral diseases, and parasitic diseases. According to literature reports, the main bacteria harmful to soft-shelled turtles are: Aeromonas, P...

Claims

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Application Information

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IPC IPC(8): A61K36/46A61P31/04
Inventor 龚卫星
Owner 长沙县明利水产养殖有限公司
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