Preparation method for eriochrome black T test paper for urinary calcium ions

Abstract

The invention discloses a preparation method for eriochrome black T test paper for urinary calcium ions. The preparation method is characterized in that the eriochrome black T test paper for urinary calcium ions is made of filter paper impregnated with eriochrome black T, triethanolamine, ammonia water, ammonium chloride, sodium dodecyl sulfate, Mg-EDTA and polyvinyl alcohol, one square centimeter of filter paper contains 0.6-2.5 mg of eriochrome black T, 0.2-1.5 mg of triethanolamine, 0.6-3.0 mg of ammonia water, 0.05-1.0 mg of ammonium chloride, 0.1-2.0 mg of Mg-EDTA and 0.5-2.0 mg of polyvinyl alcohol, and quantitative filter paper can be used as the filter paper. The eriochrome black T test paper for urinary calcium ions is convenient to carry, easy and fast to operate, visual in detection, good in selectivity, and high in accuracy and sensitivity during detection. Conditions required in the preparing process of the test paper for urinary calcium ions are easy to control, energy consumption is low, and operation is easy.

Description

technical field [0001] The invention relates to a test paper for urine detection, which belongs to the field of analytical chemistry, in particular to a preparation method and application of a test paper for detecting calcium ions in chrome black T urine. Background technique [0002] Calcium is the inorganic salt with the highest content in the human body, and it is also the most abundant mineral element in the human body. It ranks fifth in the body after oxygen, carbon, hydrogen, and nitrogen in the body. One of the characteristics of life is metabolism. Abnormal calcium metabolism will affect the physiological functions of many organs, which is very important for health. Calcium in the human body plays an important role in the transmission of nerve impulses, triggering muscle contraction, releasing hormones, and regulating heart rate: calcium deficiency can cause tetany, muscle spasm, laryngitis, convulsions, arrhythmia, and lead to bone calcification disorders, manifeste...

AI Technical Summary

Problems solved by technology

[0004] Common methods for determining urinary calcium include EDTA titration, electrode method, atomic absorption method, photometric method and test paper method, etc. Although atomic absorption spectrometry has fast determination speed, good effect and high accuracy, it requires special instruments and specialized technical operations. The cost is high, and it is difficult to apply under general laboratory conditions; the advantage of EDTA coordination titration is that it does not require special instruments, is fast and convenient, and is suitable for primary medical units, but the content of calcium in urine is low. The titration method has a large error; the electrode method The performance of the hardness electrode used is unstable and the service life is short; the methods for measuring calcium by spectrophotometry include o-cresolphthalein complex ketone direct colorimetry, micellar solubilization spectrophotometry combined calcium measurement, arsenazoⅢ color reagent Calcium method, etc.; the detection method of calcium in the human body is also developing in the direction of more convenient, fast, accurate, painless, low cost, and easy to popularize, so as to meet people's increasing requirements for medical conditions