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Application of transplantation of artificially-cultured fecal floras in treatment of intestinal diseases

A technology of intestinal diseases and fecal flora, which is applied in the field of artificially cultured fecal flora transplantation in the treatment of intestinal diseases, can solve the problems of protracted disease course, repeated attacks, and difficult to cure, and achieve no drug resistance, No toxic side effects, good therapeutic effect

Inactive Publication Date: 2015-12-30
NANCHANG UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

The course of the disease is often protracted, recurrent, difficult to cure

Method used

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Embodiment Construction

[0032] The application method of the present invention comprises the following steps:

[0033] 1. Feces collection and screening conditions:

[0034] Collect fresh feces from healthy people, seal the bags and keep them for later use; the screening conditions for feces collectors are:

[0035] a. Routine blood tests are normal;

[0036] b. Diarrhea has not occurred and antibiotics have not been used in at least the past 3-6 months;

[0037] c. No infectious diseases, such as HIV, hepatitis B, hepatitis C, etc.;

[0038] d. No history of diabetes and malignant tumors;

[0039] e. No gastrointestinal diseases;

[0040] f. No history of gastrointestinal surgery;

[0041] g. No systemic autoimmune disease or other allergic diseases;

[0042] h. No metabolic disease;

[0043] i. No bad sex habits;

[0044] j. Children, young people, partners or relatives of partners are preferred;

[0045] 2. Isolation and cultivation of fecal bacteria:

[0046] a. Take fresh feces from he...

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Abstract

Provided is application of transplantation of artificially-cultured fecal floras in treatment of intestinal diseases. According to the application, feces of healthy persons screened out under various conditions is collected, and intestinal mixed probiotic floras are collected through the steps of homogenization, suspension, dilution, filtration, centrifugation and the like; culture is performed under the anaerobic condition and the aerobic condition by the adoption of a plate isolation technology, and culturable intestinal probiotic floras are picked out; transplantation of the artificially-cultured intestinal floras is performed by the means of a gastroscope, an enteroscope, a nose-jejunum tube, fistulation, clysis and the like, intestinal flora balance is recovered, and then the effect of treating intestinal diseases is achieved. Particularly, the application has a good treatment effect on diarrhea, clostridium diffieile infection, Crohn's diseases and the like.

Description

technical field [0001] The invention relates to the application of artificially cultured fecal flora transplantation in the treatment of intestinal diseases, and realizes the treatment and protection of human intestinal diseases through the method of treating bacteria with bacteria. Background technique [0002] In the human intestinal environment, the number of intestinal flora is about 1000 to 1050, and the number of bacteria reaches 10 trillion. There are many different probiotics in the intestinal tract, including lactic acid bacteria, bifidobacteria, clostridium, faecalibacterium Genus, etc., jointly maintain the intestinal microecological balance. [0003] Many studies on human intestinal health have shown that the proportion of beneficial bacteria in the intestines of healthy people reaches 70%, that of ordinary people is 25%, and that of people with constipation is reduced to 15%, while the proportion of beneficial bacteria in the intestines of cancer patients is onl...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61K9/48A61K47/36A61P1/00
Inventor 陈廷涛辛洪波王鑫张风才黄伟华
Owner NANCHANG UNIV
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