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Bacillus amyloliquefaciens and application of protease produced by same in tanning depilation

A technology of amylolytic spores and protease, applied in the field of microbiology, can solve problems such as COD, BOD value increase, pollution, environmental pollution, etc., and achieve the effect of clear pores and smooth grain surface

Active Publication Date: 2015-12-30
SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional hair removal process, sodium sulfide and sodium hydrogen sulfide are used, and the method of hair destruction is used for hair removal, which not only produces S 2- Pollution, and increase the COD and BOD values ​​in the wastewater, the wastewater is strongly alkaline and has obvious stench, causing serious pollution to the environment

Method used

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  • Bacillus amyloliquefaciens and application of protease produced by same in tanning depilation
  • Bacillus amyloliquefaciens and application of protease produced by same in tanning depilation
  • Bacillus amyloliquefaciens and application of protease produced by same in tanning depilation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The isolation and identification of embodiment 1 bacteria

[0062] 1.1. Sample collection: soil with rotting animal hair at the outlet of the tannery sewer.

[0063] 1.2. Enrichment: Introduce the sampled samples into the enrichment medium and culture for 48 hours, then transfer to the liquid seed medium.

[0064] 1.3. Primary screening of strains

[0065] After culturing in the liquid seed medium for 12 hours, streak the bacterial suspension on the casein selective medium for 24 hours, pick single colonies with hydrolysis circles and inoculate them evenly on the casein selective medium. The colony numbers are №1-37, cultured at a constant temperature of 37°C for 48 hours, and the growth of the colonies and the hydrolysis of casein were observed.

[0066] 1.4. Re-screening of strains

[0067] Pick the colonies with obvious hydrolysis circles and evenly plant them on the solid wool medium, and cultivate them at a constant temperature of 37°C for more than 24 hours, an...

Embodiment 2K20

[0100] Embodiment 2 K20 bacterial strain physiological and biochemical performance research

[0101] 2.1. Morphological characteristics and staining results

[0102] K20 strain colony morphological characteristics: shape: round, flat. The diameter is about 2mm. Milky white, the surface is dry and smooth, and the edge of the colony is lotus leaf-shaped. See Figure 4 .

[0103] The K20 strain was rod-shaped under the microscope, and it was repeatedly proved that Gram staining was positive (see Figure 5 ). It grows well under aerobic conditions and is an aerobic bacteria. Produce spores, the spores are oval and motile.

[0104] 2.2 Physiological and biochemical characteristics detection results

[0105] Gelatin hydrolysis, aerobic growth, catalase, tyrosine hydrolysis, starch hydrolysis, nitrate reduction, and V-P reaction of K20 bacteria were all positive; phenylalanine dehydrogenase and citrate utilization were negative; available D- Glucose, D-Mannose and Maltose. ...

Embodiment 3K20

[0112] The protease depilatory performance experiment result that embodiment 3K20 bacteria produces

[0113] K20 bacteria was fermented with ② fermentation medium (%) for 72 hours, and the obtained protease-containing fermentation liquid was used for hair removal test. Wherein, ② the composition and content of fermentation medium (%) are as follows: starch: 3.0, K 2 HPO 4: 0.12, asparagine: 0.11, beef extract: 0.05, wool: 0.2pH6.4.

[0114] The experimental results show that the protease produced by K20 bacteria has good depilatory activity on sheepskin, pigskin and cowhide commonly used in tanning (Table 3): among them, the depilation effect on cowhide and sheepskin is the best. The bare skin after depilation is soft, has a softening effect, and has a smooth grain and clear pores.

[0115] The protease depilatory activity that table 3 bacterial strain produces

[0116]

[0117] +Evaluation of hair removal effect, the more +, the better the hair removal effect

[0118]...

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Abstract

The invention discloses bacillus amyloliquefaciens. The strain is preserved in the China General Microbiological Culture Collection Center on July 14th, 2015, and the preservation number is CGMCC No.11067. The invention further discloses a preparation method of the bacillus amyloliquefaciens. The invention further discloses protease produced by the bacillus amyloliquefaciens and further discloses an application of the bacillus amyloliquefaciens in tanning depilation. The protease produced by the bacillus amyloliquefaciens has good depilation activity in sheepskin, pigskin and cow leather which are frequently used in tanning, wherein the depilation effects on the cow leather and the sheepskin are the best, naked leather which is subjected to the depilation is soft, a softening effect is achieved, the grain surface is smooth, and pores are clear.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and specifically relates to a strain of Bacillus amyloliquefaciens. The invention also relates to a preparation method of Bacillus amyloliquefaciens. The invention also relates to a protease produced by Bacillus amyloliquefaciens. Application of Bacillus amyloliquefaciens in depilation of leather. Background technique [0002] The leather industry is listed as the second most polluting industry after the paper industry. In the traditional hair removal process, sodium sulfide and sodium hydrogen sulfide are used, and the method of hair destruction is used for hair removal, which not only produces S 2- pollution, and increase the COD and BOD values ​​in the wastewater, the wastewater is strongly alkaline and has obvious stench, causing serious pollution to the environment. As some small and medium-sized tanneries have been ordered to suspend or limit production in recent years, the survival ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/54C14C1/06C12R1/07
Inventor 刘彦徐祥进张东方叶永彬刘晓文宋勇峰李荣洪文卿高德良王强夏刚刘斌黄维超
Owner SICHUAN UNIV
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