Application of alpha-oxoglutarate in respect of preparing pig feed additive

A pig feed additive, ketoglutaric acid technology, applied in applications, animal feed, animal feed, etc., can solve the problems of waste of protein resources, environmental pollution, aggravation, etc., and achieve the effect of reducing healthy pig raising, reducing costs, and improving growth performance

Inactive Publication Date: 2016-01-13
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention finds that adding α-ketoglutaric acid into pig feed can significantly improve protein utilization efficiency, improve pig production performance, and reduce ammonia nitrogen discharge, thereby achieving low protein content on the premise of ensuring protein su

Method used

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  • Application of alpha-oxoglutarate in respect of preparing pig feed additive
  • Application of alpha-oxoglutarate in respect of preparing pig feed additive
  • Application of alpha-oxoglutarate in respect of preparing pig feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Effect of α-ketoglutarate on protein deposition in skeletal muscle cells

[0042] 1. In order to study the effect of α-ketoglutarate on protein deposition, skeletal muscle cells C2C12 were used as the research object. Skeletal muscle cells were seeded in three 6-well plates and divided into three groups (control group and two Treatment group) were treated with 0.5 and 2 mM α-ketoglutarate for 48 hours after being induced to differentiate into myotubes with 2% horse serum.

[0043] 2. Determination of the effect of α-ketoglutarate on the total protein concentration of skeletal muscle cells

[0044] (1) The specific measurement method is:

[0045] Cells were collected and lysed with RIPA lysate for 20 min. The lysed and broken cells were divided into 2 parts, one was used for DNA detection, and the other was centrifuged to collect supernatant for protein concentration determination. The detection method of DNA is to use the DNA kit of Magen Company to detect...

Embodiment 2

[0058] Example 2 Effect of α-ketoglutaric acid on mouse gastrocnemius muscle protein deposition

[0059] 1. In order to study the effect of α-ketoglutaric acid on protein deposition in living animals, the experiment took mice as the research object, and selected 12 3-week-old male Kunming mice (13±1g) and divided them into 2 groups after pre-feeding. Raised in single cages, one group was the blank control group, injected with normal saline through the tail vein; the other group was the treatment group, injected with 0.6 g / kg α-ketoglutaric acid through the tail vein.

[0060] The mice in the two groups were fed with the same basal diet, and had free access to water and food and natural light during the feeding period. , The gastrocnemius muscle samples of the mice were collected 3h after injection for analysis of gastrocnemius muscle protein deposition.

[0061] 2. Determination of the effect of α-ketoglutarate on protein deposition in mouse gastrocnemius muscle

[0062] (...

Embodiment 3

[0072] Example 3 Effect of α-ketoglutarate on growth performance, protein utilization efficiency and ammonia nitrogen discharge of pigs

[0073] 1. In order to study the effects of α-ketoglutarate on pig growth performance, protein utilization efficiency and nitrogen discharge, 180 nursery pigs with an average age of 30 days and an average weight of 7.76kg were selected and randomly divided into 4 treatments , 3 replicates per treatment, 15 pigs per replicate.

[0074] (1) Control group: fed a basal diet with a crude protein level of 18.48%, where the source of crude protein was corn, soybean meal, fish meal or other miscellaneous meal-type diets;

[0075] (2) Low protein group: on the basis of the basal diet of the control group, the protein level was reduced by 1%;

[0076] (3) 0.3% α-KG group: Add 0.3% α-ketoglutaric acid (α-KG) on the basis of the low protein group (that is, the content of α-KG is 3kg / ton of pig feed);

[0077] (4) 0.5% α-KG group: Add 0.5% α-ketogluta...

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Abstract

The invention discloses application of alpha-oxoglutarate in the respect of being used as or preparing a pig feed additive and a low-protein pig feed containing the alpha-oxoglutarate. The pig feed additive can improve the utilization efficiency of protein in a pig feed and the production properties of pigs, and can reduce the discharge of ammonia and nitrogen. Through researches of the application disclosed by the invention, the alpha-oxoglutarate is added to the pig feed, so that the protein utilization efficiency of the pig feed and the production properties of the pigs can be obviously improved, under the premise that the protein supply is guaranteed in the process of raising the pigs, the preparation of the pig feed with a low protein content is realized, the cost of the pig feed is reduced, technology support and cost support are provided for adding superior protein raw materials to the pig feed, and the problems of the waste of protein resources and the environment pollution are also solved; in addition, the alpha-oxoglutarate does not contain substances which are toxic and harmful to the growth and the health of pigs, the alpha-oxoglutarate can be directly added to a premix, the addition process is convenient and swift, the preparation method is simple, requirements for special equipment do not exist, and the alpha-oxoglutarate has good popularization and application prospects.

Description

technical field [0001] The invention belongs to the technical field of feed additives. More specifically, it relates to the application of α-ketoglutarate in the preparation of pig feed additives and pig feed containing α-ketoglutarate. Background technique [0002] The sustainable development of my country's pig industry is related to the national economy and people's livelihood, but the shortage of protein feed resources and nitrogen emission pollution are the bottlenecks restricting the sustainable development of my country's pig industry. In the current pig production in my country, on the one hand, due to cost factors, the use of high-quality protein raw materials has to be reduced, resulting in limited animal growth; Efficiency decreases, ammonia nitrogen excretion increases, but causes waste of protein resources and aggravation of environmental pollution. Therefore, it is very important to develop new feed additives to ensure that the growth performance of animals ca...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K20/105A23K10/22A23K10/30
Inventor 束刚江青艳蔡兴才朱晓彤王丽娜
Owner SOUTH CHINA AGRI UNIV
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