SCAP gene related to lactation of buffalo, and application of same as molecular marker

A molecular marker, buffalo technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem that there is no buffalo SCAP gene, etc., and achieve high fat-free solid content, high total dry matter content, and high lactose rate Effect

Active Publication Date: 2017-07-21
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there is no research report on the buffalo SCA

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SCAP gene related to lactation of buffalo, and application of same as molecular marker
  • SCAP gene related to lactation of buffalo, and application of same as molecular marker
  • SCAP gene related to lactation of buffalo, and application of same as molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0055] Example 1: Preparation of Buffalo Genome Total DNA

[0056] The total genomic DNA of buffalo (Tiangen, Beijing) was prepared by using the blood genomic DNA extraction kit. The specific operation steps are as follows:

[0057] (1) Take 500 μL of whole blood and place it in a 1.5 mL centrifuge tube, add an equal volume of cell lysis solution CL, invert and mix, centrifuge at 12,000 rpm for 1 min, aspirate the supernatant, and leave the precipitate; add an equal volume of cell lysis solution CL, repeat this process Step once; add 4 μL RNAase (100 mg / mL), shake for 15 sec, and let stand for 5 min at room temperature;

[0058] (2) Add 20 μL Proteinase K solution, mix well, add 200 μL Buffer GB, fully invert and mix, place at 56°C for 10 min, invert and mix several times during this period, and the solution should become clear;

[0059] (3) Add 200 μL of absolute ethanol, invert and mix well; flocculent precipitation may occur at this time;

[0060] (4) above-mentioned gain...

Example Embodiment

[0066] Example 2: Acquisition of SCAP gene molecular markers for buffalo milk production traits

[0067] 1. Primer design: Using SEQ NO: 1 as the template, using Primer5.0 primer design software, and detected by Oligo software, the forward primer for amplifying the buffalo SCAP gene was determined, and entrusted to Dalian Bao Biotechnology Co., Ltd. to synthesize, of which The DNA sequences of the forward and reverse primers used to amplify the gene are shown in SEQ ID NOs: 2-5.

[0068] 2. PCR amplification reaction

[0069] Fifty buffalo genomic DNA samples were randomly selected for pooling as templates for PCR reactions.

[0070] (1) PCR reaction: The total reaction volume is 40 μL, including 1 μL of buffalo DNA mixed pool template, 20 μL of 10×LA Taq Mix, 2 μL of primer mix (10 mM each) and ddH 2 O 17 μL.

[0071] (2) PCR reaction program: 94°C / 3min; 94°C / 30sec, 60°C / 30sec, 72°C / 1.5min, 30 cycles; 72°C 10min; storage at 4°C

[0072] 3. PCR product sequencing, analysis...

Example Embodiment

[0088] Example 3: Gene SCAP related to buffalo lactation and its application as a molecular marker

[0089] The breed of the test buffalo herd was a hybrid buffalo herd. The association analysis of different genotypes of SCAP gene A1717600G, G1718168A, G1743088A, T1753656C, G1759116A, C1759142T, G1760740A, A1762368G, T1766036C loci in buffalo herds and buffalo milk production traits was performed.

[0090] Table 2 shows the results of association analysis of buffalo SCAP genes A1717600G, G1718168A, G1743088A, T1753656C, G1759116A, C1759142T, G1760740A, A1762368G and T1766036C. From Table 2, it can be concluded that genotype was significantly correlated with buffalo milk production traits (P<0.05).

[0091] For the A1717600G locus, the milk production of buffalo individuals with GA genotype was higher than that of AA and GG genotypes.

[0092] For the G1760740A locus, the milk production of buffalo individuals with GG genotype was higher than that of AA and GA genotypes.

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a SCAP gene related to lactation of a buffalo, and application of the same as a molecular marker, belonging to the field of molecular markers for livestock. The nucleotide sequence of the gene is as shown in SEQ ID No. 1, wherein 9 mutant bases are respectively located at site 1717600 bp, site 1718168 bp, site 1743088 bp, site 1753656 bp, site 1759116 bp, site 1759142 bp, site 1760740 bp, site 1762368 bp and site 1766036 bp, and the 9 SNP sites are all substantially related to the milk production properties of the buffalo. The invention discloses the sequences of upstream and downstream primers needed by all the base mutation sites in SEQ ID No. 1 in a detection sequence table. Application of the SCAP gene is beneficial for improving the milk production performance of the buffalo and provides technical support and theoretical foundation for breeding of milk buffalos with excellent milk production performance.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field, in particular to the field of animal molecular markers. It specifically relates to the gene SCAP related to buffalo lactation and its application as a molecular marker. [0003] 【Background technique】 [0004] Buffalo is a unique breeding stock resource in southern my country, but its low milk yield and reproductive performance have become two major scientific problems affecting its industrial development. Obviously, the use of advanced molecular marker technology in the selection of high-quality milk buffalo germplasm resources will effectively improve the efficiency of selection. [0005] Milk production trait is an important economic trait of milk buffalo, which is determined by a variety of microgenes and is a typical quantitative trait controlled by polygenes. Clone milk buffalo lactation performance-related genes and explore their genetic characteristics to improve milk buffalo production pe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/12C12Q1/68
CPCC07K14/47C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 邓廷贤梁贤威庞春英马小娅梁莎莎朱鹏陆杏蓉段安琴
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap