A method for inducing adventitious buds from leaves of Gesneria oleracea by intermittent immersion

Abstract

The invention discloses an intermittent immersion type gesneriaceae leave adventitious buds induction method. The method comprises the following steps: (1) sterilizing the surface of gesneriaceae plant leaves; (2) inoculating the surface sterilized gesneriaceae plants in a solid basic medium to cultivate for 7-10 days for sterile explant screening; and (3) placing non-pollution gesneriaceae plant leaves in a communication vessel type intermittent plant tissue cultivation device for intermittent immersion type cultivation. The method has the advantages that an utilization rate of cultivation space is high and exchange of cultivate gas components is facilitated, so that quality and propagation coefficients of cultivated substances are improved, being suitable for large-scale production; the cultivation device and accessories are easy for integral disinfection and sterilization, the cultivation process is totally closed, and contamination probability is little; the structure of the cultivation device is simple and compact, energy consumption is low, cultivation space is saved, and a tissue cultivation cost is reduced; and transfer subculture operation of tissue cultivated seedlings in the whole tissue cultivation process is simplified, labor and production cost are saved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to an intermittent submerged method for inducing adventitious buds from leaves of chicory moss. Background technique [0002] Plant tissue culture technology has the advantages of large and fast reproduction, easy control of experimental conditions, continuous production, and high production efficiency. It is widely used in crop breeding, germplasm resource preservation, and virus-free seedlings of flowers, fruit trees, vegetables, forest trees, and Chinese herbal medicines. Production and rapid propagation and vegetative propagation, such as the vegetative rapid propagation of lily virus-free seed balls. [0003] Traditional solid or semi-solid tissue culture methods consume a large amount of agar, and a large number of containers need to be handled during filling and cleaning, which consumes a lot of labor and leads to high production costs. [0004] At present, some p...

AI Technical Summary

Problems solved by technology

However, such devices are usually bulky, difficult to disassemble or move, and the control elements are complicated, and the maintenance cost is high, and they are not easy to operate aseptic manual inoculation and efficient overall cleaning and sterilization of the device

[0005] Existing domestic use leaves as explants to carry out the report of intermittent immersion type culture very few, the patent No. CN102150624A discloses a kind of method of pinellia leaf tissue culture technology to induce cluster buds, although introducing intermittent type in the cultivation process Immersion culture technology, but the most important intermittent immersion culture device has not been introduced and improved, ignoring the consideration of the most important limiting factor in the entire culture step, that is, the intermittent immersion culture device, which affects leaf proliferation to a certain extent Improvement of rapid propagation efficiency

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