Microbial ecological agent for preventing and treating piglet diarrhea and preparation method of microbial ecological agent

A micro-ecological preparation, piglet diarrhea technology, applied in the directions of medical preparations, pharmaceutical formulations, drug combinations, etc. containing active ingredients, can solve the problems of limited promotion and application, complex production procedures, easy inactivation of strains, etc., to prevent piglets. The effect of diarrhea, simple production process and low production cost

Inactive Publication Date: 2016-02-24
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are unfavorable factors such as complex production procedures, higher production costs than antibiotics, and easy inactivation of strains during transportation and storage, which limit the promotion and application of such probiotics to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of probiotics is:

[0024] 1) The feces of Guizhou Congjiangxiang piglets growing healthy and on the eighth day after weaning were collected and stored in liquid nitrogen at low temperature. When preparing pig feces bacteria liquid, the pig feces were quickly thawed in a 37°C water bath, and then added to sterile saline to make a suspension. Filter the suspension successively with a stainless steel tea strainer and a cell sieve (0.224 mm in aperture) to obtain pig feces bacteria liquid. The live bacteria in the pig feces liquid was counted by the live bacteria counting method, and the live bacteria content in the pig feces liquid was adjusted to 10 according to the counting results. 4 ~10 11 each milliliter, then add glycerol to swine feces bacterial liquid to obtain swine feces bacterial liquid cryopreservation liquid (volume fraction of glycerol is 10%), place the cryopreservation in liquid nitrogen.

[0025] Wherein, the pig feces liquid con...

Embodiment 2

[0029] The preparation method of probiotics is:

[0030] 1) The feces of Guizhou Congjiangxiang piglets growing healthy and on the eighth day after weaning were collected and stored in liquid nitrogen at low temperature. When preparing pig feces bacteria liquid, the pig feces were quickly thawed in a 37°C water bath, and then added to sterile saline to make a suspension. Filter the suspension successively with a stainless steel tea strainer and a cell sieve (0.224 mm in aperture) to obtain pig feces bacteria liquid. The live bacteria in the pig feces liquid was counted by the live bacteria counting method, and the live bacteria content in the pig feces liquid was adjusted to 10 according to the counting results. 4 ~10 11 each milliliter, then add glycerol to swine feces bacterial liquid to obtain swine feces bacterial liquid cryopreservation liquid (volume fraction of glycerol is 10%), place the cryopreservation in liquid nitrogen.

[0031] Wherein, the pig feces liquid con...

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PUM

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Abstract

The invention discloses a microbial ecological agent for preventing and treating piglet diarrhea and a preparation method of the microbial ecological agent. The microbial ecological agent is prepared from pig manure, is used for preventing and treating piglet diarrhea, and has obvious effects, and thus the use amount of an antibiotic for the pig is reduced in the prevention and treatment of the piglet diarrhea. The preparation method comprises the following steps: firstly collecting the manure of the healthy fragrant pig from the Congjiang County of Guizhou Province, adding the manure into stroke-physiological saline solution to prepare a suspension, filtering the suspension with a stainless steel tea hopper and a cell sieve (the hole diameter is 0.224mm) sequentially, thus obtaining the manure bacterial liquid of the pig, adding a strain protection agent to the manure bacterial liquid of the pig, and uniformly mixing, and thus obtaining the microbial ecological agent, wherein 10<3>-10<10> viable organisms exist in each milliliter of the microbial ecological agent. During use, the microbial ecological agent is drenched to the stomach of the piglet, wherein the drenching dosage is 1-10 ml each time, once per day. The preparation process of the microbial ecological agent is simple, the production cost is relatively low, the microbial ecological agent has the significant effect for preventing and treating piglet diarrhea, and the growth performance of the piglet is not influenced.

Description

technical field [0001] The invention relates to a biological preparation for preventing and treating piglet diarrhea, in particular to a microecological preparation for preventing and treating piglet diarrhea and a preparation method thereof. Background technique [0002] Due to the immature development of the immune system, piglets are prone to diarrhea when they are stimulated by the outside world. Especially the early weaning measures of piglets will stimulate the piglets from the aspects of nutrition, psychology and environment, which will lead to early weaning stress in piglets, and cause diarrhea or even death in piglets in severe cases. Antibiotics are widely used in production practice to alleviate diarrhea in piglets, but with the increasingly prominent problems of antibiotic resistance and food safety, banning antibiotics has become an inevitable trend. [0003] At present, probiotics are commonly used to partially replace the use of antibiotics for pigs with prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/24A61K35/74A61K36/06A61P1/12
Inventor 晏向华胡军聂杨帆
Owner HUAZHONG AGRI UNIV
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