Detection method of city wastewater treatment plant/station microbial aerosol

Abstract

The invention discloses a detection method of city wastewater treatment plant / station microbial aerosol. The detection method includes the steps of firstly, using a medium-flow portable atmosphere sampler to collect the microbial aerosol of city wastewater treatment plant / station test points, and collecting and sampling the meteorological parameters at the moment; secondly, eluting particles on a sampling membrane, and extracting the total DNA of the particles; thirdly, performing target fragment amplification and high-throughput sequencing; fourthly, performing sequence analysis to identify the community structure of the microbial aerosol; fifthly, analyzing the diversity and similarity of the community structure of the microbial aerosol; sixthly, analyzing the dissipation features and sources of the microbial aerosol. The method is a feasible method for extracting trace DNA in the city wastewater treatment plant / station microbial aerosol, and a fundamental premise and foundation is provided for the analysis of the dissipation features and diversity of the microbial aerosol.

Description

technical field [0001] The invention relates to the technical field of environmental microbial analysis, in particular to a method for detecting and analyzing all microorganisms in microbial aerosols in urban sewage treatment plants / processing stations. Background technique [0002] Bioaerosols refer to living aerosol particles (including microbial particles such as bacteria, fungi, and viruses) and active particles (pollen, spores, etc.) and various plasmids released into the air by living organisms. A variety of microorganisms exist in sewage. In the process of sewage treatment, aeration, mechanical stirring and sludge dehydration can release them into the surrounding air in the form of aerosols. Studies have shown that microbial aerosols emitted from urban sewage treatment plants / treatment stations contain a large number of pathogenic bacteria or opportunistic pathogens, which have great potential for the health of workers in sewage treatment plants / sewage treatment stati...

AI Technical Summary

Problems solved by technology

Obtaining the DNA of microbial aerosols is the prerequisite for the subsequent analysis of microbial characteristics. However, the number of microorganisms in the air is much lower than that of other habitats (soil, water), and it has been a long-standing practice to directly extract DNA from microorganisms in the air without culturing. problem

[0006] The SKC-Sampler liquid sampler has a small flow rate (rated flow rate is 12.5L air / min), and it takes a long time for sample collection to obtain sufficient sample volume to extract DNA; the microbial aerosol collection solution (PBS buffer) in this method is in the sample A large amount of volatilization will occur during the collection process, and the collection liquid needs to be added repeatedly to ensure its normal operation; at the same time, due to the fact that the collection liquid will freeze under low temperature conditions (<0°C), this method cannot complete the sample collection task at any time

[0007] Refer to the common large-flow air sampler (flow > 100L / min), the air sampler is generally installed at a fixed sampling point, and for the investigation arrangement between different sewage treatment plants / processing stations, and between multiple sampling points frequently Replacement requirements are not met

[0008] The above shortcomings make the two international standard bioaerosol collection methods unable to achieve the purpose of on-site sampling of all microorganisms in the microbial aerosol of urban sewage treatment plants, as well as the subsequent analysis of microbial community structure and escape characteristics

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