Detection method of city wastewater treatment plant/station microbial aerosol

A technology of urban sewage and detection methods, which is applied in the field of environmental microbial analysis, can solve the problems of frequent replacement requirements, difficulty in extracting DNA, and inability to complete sample collection tasks, etc.

Inactive Publication Date: 2016-03-02
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

Obtaining the DNA of microbial aerosols is the prerequisite for the subsequent analysis of microbial characteristics. However, the number of microorganisms in the air is much lower than that of other habitats (soil, water), and it has been a long-standing practice to directly extract DNA from microorganisms in the air without culturing. problem
[0006] The SKC-Sampler liquid sampler has a small flow rate (rated flow rate is 12.5L air/min), and it takes a long time for sample collection to obtain sufficient sample volume to extract DNA; the microbial aerosol collection solution (PBS buffer) in this method is in the sample A large amount of volatilization will occur during the collection process, and the collection liquid needs to be added repeatedly to ensure its normal operation; at the same ...

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  • Detection method of city wastewater treatment plant/station microbial aerosol
  • Detection method of city wastewater treatment plant/station microbial aerosol
  • Detection method of city wastewater treatment plant/station microbial aerosol

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Embodiment 1

[0043] like figure 1 Shown, a kind of detection method of urban sewage treatment station (WWTS) microbial aerosol, comprises steps as follows:

[0044] 1) Preparation of the sampler and its accessories, preparation of the sampling film and its collection device, and high temperature and high pressure sterilization;

[0045] 2) On-site collection of microbial aerosols in urban sewage treatment plants, arrangement of sampling points, operation of air sampling pumps, suction and filtration of air onto sterile glass fiber filter membranes, and retention of airborne microorganisms on the sampling membranes;

[0046] 3) Collection and storage of the sampling film. Use sterile tweezers to transfer the sampling film that traps airborne microorganisms to sterile aluminum foil and seal it. If the sample cannot be processed immediately, store it in a freezer at -20°C or -80°C. Store in the refrigerator;

[0047] 4) DNA extraction, extracting the microbial DNA on the glass fiber membra...

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Abstract

The invention discloses a detection method of city wastewater treatment plant/station microbial aerosol. The detection method includes the steps of firstly, using a medium-flow portable atmosphere sampler to collect the microbial aerosol of city wastewater treatment plant/station test points, and collecting and sampling the meteorological parameters at the moment; secondly, eluting particles on a sampling membrane, and extracting the total DNA of the particles; thirdly, performing target fragment amplification and high-throughput sequencing; fourthly, performing sequence analysis to identify the community structure of the microbial aerosol; fifthly, analyzing the diversity and similarity of the community structure of the microbial aerosol; sixthly, analyzing the dissipation features and sources of the microbial aerosol. The method is a feasible method for extracting trace DNA in the city wastewater treatment plant/station microbial aerosol, and a fundamental premise and foundation is provided for the analysis of the dissipation features and diversity of the microbial aerosol.

Description

technical field [0001] The invention relates to the technical field of environmental microbial analysis, in particular to a method for detecting and analyzing all microorganisms in microbial aerosols in urban sewage treatment plants / processing stations. Background technique [0002] Bioaerosols refer to living aerosol particles (including microbial particles such as bacteria, fungi, and viruses) and active particles (pollen, spores, etc.) and various plasmids released into the air by living organisms. A variety of microorganisms exist in sewage. In the process of sewage treatment, aeration, mechanical stirring and sludge dehydration can release them into the surrounding air in the form of aerosols. Studies have shown that microbial aerosols emitted from urban sewage treatment plants / treatment stations contain a large number of pathogenic bacteria or opportunistic pathogens, which have great potential for the health of workers in sewage treatment plants / sewage treatment stati...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6869
Inventor 韩云平许光素刘俊新
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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