Flowering period synchronization regulating method for wheat and corn cross inducing haploid embryo

Abstract

The invention discloses a flowering period synchronization regulating method for wheat and corn cross inducing haploid embryo. The method comprises the steps that on April 8-18 of every year, 200 materials of a single crossing and multiple crossing F1 generation and a preferable F2 generation of wheat are prepared, seeds are harvested after the wheat is ripe, each material contains 10 ears, threshing and drying are performed, unripened grains, extremely-small grains and grains damaged by diseases and pests are removed, the grains are stored in a seed refrigerating chamber and prepared to be applied to a staging autumn sowing test; from June to October, 4-8 corn species of different types are selected to serve as corn seeds for the test, the seeds are stored in the seed refrigerating chamber and prepared to be applied to the staging test. The wheat flowering period and the corn flowering period are regulated to be synchronized in mid-to-end April, the flowering period is regulated through the methods such as seed sorting, fertilizer and water and disease, pest and weed damage prevention and control management enhancing and staging temperature controlling, therefore, plant health and strongness of the wheat and corn can be promoted, the wheat and corn cross pollination time can be prolonged, the flowering period synchronization regulating successful rate is increased, and light supplementary is performed on a greenhouse by controlling heat increasing in a cold chamber; the equipment is simple, the effect is good, and the operation cost is low.

Description

Technical field [0001] The invention relates to a regulating method for producing wheat haploid embryos, in particular to a method for regulating the flowering period of haploid embryos induced by the hybridization of wheat and corn, which belongs to one of the key links of wheat haploid technology breeding. Background technique [0002] Maize pollen inducing wheat haploid technology has become a more efficient way to produce wheat haploid through more than 20 years of domestic research. Wheat haploid has important application value in screening gene mutants and gene function research. Ploidy has important application value in gene mapping, construction of linkage genetic map, fixing heterosis, and accelerating the process of wheat breeding. [0003] Using the high embryo rate of wheat × corn and the spontaneous elimination of corn chromosomes in heterozygotes, wheat haploids can be obtained. Due to the lack of endosperm and abortion, the immature embryos die in the early stages o...

AI Technical Summary

Problems solved by technology

Under normal circumstances, the flowering period of wheat is in the middle and late April, and the flowering period of corn is from June to September. Under conventional cultivation conditions, the flowering period of the two cannot meet; it is difficult to ensure the vitality of corn pollen by storing rice pollen in liquid nitrogen for wheat pollination, resulting in failure Guaranteed to obtain haploid embryos, so adjusting the flowering period of wheat and corn to meet in mid-to-late April is one of the key links in the application of wheat-corn hybridization induction wheat haploid technology to wheat breeding