Traditional Chinese medicine composition for treating colon cancer
A composition and technology of colon cancer, applied in the field of medicine, can solve the problems of difficult clinical treatment, and there is no special effective method to completely cure colon cancer, and achieve significant curative effect, no toxic side effects, and high efficiency
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Embodiment 1
[0015] Weigh 10 grams of cat ginseng, 12 grams of milk seed root, 15 grams of hawthorn root, 12 grams of grape root, 12 grams of red vine, 15 grams of flying crane, 10 grams of Hedyotis diffusa, 12 grams of Resurrection Grass, and 10 grams of vine pear root. 10 grams of Buddleia, 12 grams of Plantago, 10 grams of Hongmuxiang, 10 grams of Baimuxiang, 15 grams of Bamboo Leaf, 12 grams of Panax notoginseng, 15 grams of Polygonum cuspidatum, 10 grams of Atractylodes macrocephala, 10 grams of Tiankui Seed, 12 grams of Chuanxiong 10 grams of Danshen, 10 grams of Daqingye, 10 grams of barbata, 8 grams of pearl umbrella, 12 grams of lobelia, 10 grams of single flower, 12 grams of seven leaves, 12 grams of baby vine, mountain conch 10 grams and 10 grams of thistle, crushed, added 100,000 ml of water, heated under reflux for 3 hours, filtered, the filtrate was cooled to room temperature, and then filtered through a 0.22 μm microporous membrane to obtain solution 1.
Embodiment 2
[0017] Weigh out 0.1 g of Add 80,000 ml of sterile water, sonicate for 30 minutes, and then filter through a 0.22 μm microporous membrane to obtain solution 2.
experiment example
[0019] MTT (purchased from Sigma Company) was dissolved in normal saline, prepared into a working solution of 5 mg / mL, and stored at -20°C; dimethylsulfoxide (DMSO) was produced by Xi'an Chemical Reagent Factory and was analytically pure.
[0020] Principle of MTT method
[0021] MTT is an acceptable H + yellow dye. In the mitochondrial respiratory chain of living cells, succinate dehydrogenase and cytochrome C can cleave the tetrazolium ring of MTT to produce blue-purple formazan crystals. Dead cells do not contain this dehydrogenase. The amount of Formazan crystals generated is directly proportional to the number of viable cells, and the DMSO solution of the crystals has a maximum absorption peak at 570nm. Therefore, the effect of the drug on cell proliferation can be evaluated by detecting the amount of formazan crystals.
[0022] testing method
[0023] Take the tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 5 cells / mL, add 200 μL per ...
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