A kind of fermentation culture method of staghorn ganoderma lucidum
A technology for fermentation and cultivation of Ganoderma lucidum, applied in the field of microorganisms, can solve the problems of long cycle, low production efficiency, large environmental impact, etc., and achieve the effect of increasing yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] Example 1 A method for liquid culture of Staghorn Ganoderma lucidum, the specific steps are as follows:
[0031] (1) Maternal amplification
[0032] Under aseptic conditions, dissect a small piece of Staghorn Ganoderma lucidum mother bell, transfer it to a slant medium, stopper, put it in a constant temperature incubator, and cultivate it at 27°C for 7 days;
[0033] Slant medium: Wash potatoes, peel them, weigh 200g, cut into small pieces, and 30g wheat bran, add 1000mL water to boil, keep for 30min, filter, add 20g agar to the filtrate, heat it to melt, add 30g glucose , 2g potassium dihydrogen phosphate, 1g magnesium sulfate, pH 7.0, put them into test tubes, put them in an autoclave for sterilization, at a temperature of 121°C, for 30 minutes, and make a test tube slope (18mm×180mm) for use;
[0034] (2) Seed liquid preparation
[0035] Take 1cm on the slant medium 2 The mycelium was inoculated into the seed liquid culture medium and placed in a shaker at 27°C and 150r / min f...
Embodiment 2
[0040] Example 2 Evaluation of the ability of Staghorn Ganoderma lucidum to produce Ganoderma lucidum polysaccharides and triterpenoids
[0041] Staghorn Ganoderma lucidum strain NCPSLZ1 is selected, and the deposit number of strain NCPSLZ1 is CCTCCNo.M2015796; the preservation unit is the Chinese Type Culture Collection; the preservation date is December 29, 2015; the preservation address is Wuhan University, Wuhan, China.
[0042] The liquid fermentation medium used in the evaluation. The medium formula (calculated by mass percentage) is 15% potato juice, 3% wheat bran, 3% glucose, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and pH 7.0 . The inoculum amount is 5% (V / V), 500mL shake flask (250mL liquid), 150r / min, 27±2℃ constant temperature culture for 70h to obtain fermentation broth for evaluation, and further to obtain freeze-dried mycelium powder.
[0043] The mycelium biomass of the strain NCPSLZ1 was determined by the dry weight method: the fermentation broth...
Embodiment 3
[0047] Example 3 Optimization of liquid fermentation conditions
[0048] 3.1 Maternal amplification
[0049] Wash potatoes, peel them, weigh 200g, cut into small pieces, and 30g wheat bran, add 1000mL water to boil, keep for 30min, filter, add 20g agar to the filtrate, heat to melt all, add 30g glucose, dihydrogen phosphate Potassium 2g, magnesium sulfate 1g, pH 7.0, divided into test tubes, put into an autoclave for sterilization (temperature 121°C, time 30min), made a test tube slope (18mm×180mm) for later use.
[0050] Under aseptic conditions, dissect the mother seed of a small piece of strain NCPSLZ1, transfer it to the slant medium, plug it, and place it in a constant temperature incubator, cultivate at 27°C. After the hyphae grows over the slant, place the expanded mother seed Store in a refrigerator at 4°C until use.
[0051] 3.2 Seed liquid preparation
[0052] 15% potato juice, 3% wheat bran, 3% glucose, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, pH 7.0, th...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More