A kind of breeding method of the fourth generation of sunflower inbred line Anniversary

An inbred line, sunflower technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of weak combination advantages, long breeding cycle, shortage, etc., and achieve the effect of shortening the cultivation cycle

Active Publication Date: 2018-07-17
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, my country's sunflower variety resources and inbred line materials used to assemble three-line hybrids are relatively scarce, with a narrow genetic basis and weak combination advantages. Wild sunflower resources and local varieties are mainly used to hybridize with existing backbone inbred lines. Breeding inbred lines with high quality, disease resistance and wide adaptability, and the use of wild sunflowers often have problems such as self-incompatibility and difficulty in species conservation. In terms of breeding methods, due to limited light and heat resources in the north, breeding materials can only Plant one generation, and then use winter breeding in Hainan to realize the existing two-generation breeding mode of sunflower inbred lines every year. Therefore, it takes at least 3 years to breed a new sunflower inbred line. The breeding process is slow, so speeding up the utilization of high-quality sunflower resources and the selection of excellent sunflower inbred lines to cultivate sunflower inbred lines with excellent traits, disease resistance, and high combining ability are of great significance for enriching the genetic population of sunflower breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The concrete steps of the sunflower inbred line anniversary four-generation breeding method that the present invention adopts are as follows:

[0022] (1) The first generation of spring sowing breeding: The selection criteria for spring sowing breeding sites is based on the effective accumulated temperature of ≥5°C within 110 days from the date of sowing ≥1900°C; the seedlings are raised in greenhouse pots in early March, and transplanted to the breeding test field after 30-35 days. Irrigate and fertilize to ensure that the seedlings are neat and strong; in the first ten days of May, the seedlings are bagged for pollination, and 10 days after pollination, the embryos are manually picked up for inoculation and culture, and the first generation is completed.

[0023] (2) The second generation of summer sowing breeding: The selection criteria for summer sowing breeding sites is based on the effective accumulated temperature of ≥5°C within 100 days from the date of embryo in...

Embodiment 2

[0028] A breeding method for the fourth generation of sunflower inbred lines. The first generation is bred in spring in Shihezi City, Xinjiang. On the basis of spring sowing, the second generation is bred by using the technology of young embryo culture in summer; For the breeding of two generations of sunflower inbred lines, the goal of four-generation breeding is achieved. The specific steps of the breeding method are as follows:

[0029] (1) The first generation of transplanting and breeding in spring sowing greenhouse seedlings in Shihezi City, Xinjiang: Seedlings were raised in pots and pots in the greenhouse on March 15, and transplanted to the breeding test field after 30-35 days, watered and fertilized to ensure that the seedlings were neat and strong, and on May 15 The left and right flowers are bagged for pollination, and 10 days after pollination, the embryos are manually picked up for inoculation and culture, that is, the first generation of addition is completed.

...

Embodiment 3

[0034] Using the serial breeding experiments of the fourth generation of sunflower inbred lines in the series of the above-mentioned embodiments of the present invention, 6 generations have been added continuously from March 2013 to September 2014, and stable sunflower restorer lines Y2592R and Y2613R have been bred. The restorer lines are Branch type, plant height 80-90 cm, large amount of pollen, long oval grain, 100-grain weight about 3g, good appearance, good commercial performance, disease resistance, growth period of about 95 days, hybrids XKY1501 and XKY1502 have been configured, thus It can be seen that this method significantly shortens the selection process of sunflower inbred lines, reduces the cost of selection, and has outstanding comprehensive advantages.

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Abstract

The invention discloses a breeding method of anniversary four generations of a sunflower selfing line. The biological characteristics of sunflowers, the sunflower selfing line research and current breeding selection situations are utilized, the young embryo breeding technology and the greenhouse seedling breeding technology are combined with field experiment planting, with sunflower field breeding planting as the standard, greenhouse planting is combined, effective accumulative temperature, higher than or equal to 5 DEG C, needed by completing one generation of the sunflower selfing line and illumination are explored, a planting place is selected reasonably, advanced technical means and methods are utilized as technology core guarantees, in 7-10 days after fertilization, embryos are taken out and bred, and the next generation begins to be bred, so that the generation cultivation period is shortened, the breeding method of the anniversary four generations of the sunflower selfing line is set up, the breeding cost is reduced, and the method has a realistic function and significance in the technical field of breeding of the sunflower selfing line.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to the technical field of breeding of sunflower inbred lines. Background technique [0002] sunflower( Enthusiast annuls L. ) is one of the five major oil crops in the world. It has the characteristics of resistance to salt and alkali, drought and barrenness. It is also the main oil crop in northern my country. It is increasingly valued as an oil or protein resource. There are quite a few dry and barren low-yield fields and saline-alkali land in China, so sunflower plays an important role in agricultural production. All countries in the world have generally carried out research on three lines (sterile line, maintainer line, restorer line) in the study of sunflower heterosis utilization. my country has carried out research on three-line sunflower breeding since the 1970s. [0003] At present, my country's sunflower variety resources and inbred line materials used to assem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 刘胜利段维王鹏柳延涛赵刚李万云刘建喜周玉堂
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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