Multi-channel microfluidic photoelectric detection device and method for detection of various bacterial species
A photoelectric detection and multi-channel technology, applied in the field of microbial detection, can solve the problems of narrow application range of detection and achieve the effects of wide application detection range, convenient operation, high degree of integration and automation
Active Publication Date: 2018-10-09
宁波爱基因科技有限公司
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For the microbial detection device, the Chinese patent application number CN201010284474.5 proposes a method for rapid detection of Escherichia coli using a microfluidic chip and the corresponding microfluidic chip, but the microfluidic chip can only target one The microbial detection object is tested, the detection object is unique, and the scope of detection is narrow
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[0024] see figure 1 In the shown multi-channel microfluidic photoelectric detection device for the detection of various strains, the microfluidic chip 9 is placed horizontally, and a plurality of mixing reaction channels 4 are arranged on the microfluidic chip 9 . Directly below the microfluidic chip 9 is a stepping motor 13, which is vertically arranged, and its output shaft is fixedly connected to the center of the microfluidic chip 9 to drive the microfluidic chip 9 to rotate.
[0025] A light beam emitter 24 is arranged above the microfluidic chip 9, and a plurality of photomultiplier tubes 23 are arranged below the microfluidic chip 9. The numbers are the same, one photomultiplier tube 23 is located just below one mixed reaction pipeline 4 , and the photomultiplier tube 23 corresponds to the mixed reaction pipeline 4 one by one. The beam emitter 24 is fixedly connected below a horizontal movement mechanism, and the horizontal movement mechanism drives the beam emitter 24...
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The invention discloses a multi-channel microfluidics photoelectric detection device and method for detecting various strains of microorganisms. A plurality of mixed reaction pipes are arranged on a microfluidics chip; a light beam emitter is arranged above the microfluidics chip; a plurality of photomultipler tubes are arranged below the microfluidics chip; the light beam emitter is fixedly connected to the lower part of a horizontal moving mechanism; a stock solution pool is arranged in the middle of the microfluidics chip; a plurality of fan-shaped mixed reaction pipes are arranged on the periphery of the stock solution pool; a chemical reagent box is arranged at an initial end of each mixed reaction pipe; the initial end of each mixed reaction pipe is connected to the stock solution pool through a stock solution delivering pipe; and the microfluidics chip is used for mixing liquid to be detected and a chemical reagent by using a rotary centrifugal mixing technology. In a photoelectric detection process, an automatic moving type light source is designed to accurately detect reaction channels one by one, various strains of the microorganisms in the liquid to be detected can be detected simultaneously, and integration and automation degree are high.
Description
technical field [0001] The invention belongs to the field of microorganism detection, and in particular relates to a microfluidic photoelectric detection technology for detecting multiple strains of microorganisms. Background technique [0002] Microbiological detection plays a decisive role in food processing, clinical diagnosis, industrial wastewater treatment, and preparation of agricultural culture fluid. The application of microfluidic chips in the field of microbial detection has also begun to develop rapidly. For the microbial detection device, the Chinese patent application number CN201010284474.5 proposes a method for rapid detection of Escherichia coli using a microfluidic chip and the corresponding microfluidic chip, but the microfluidic chip can only target one The microbial detection object is tested, the detection object is unique, and the scope of application of the detection is narrow. Contents of the invention [0003] Aiming at the deficiencies of the c...
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IPC IPC(8): G01N21/82
CPCG01N21/82G01N2021/825
Inventor 徐佩锋张元霞杨宁张荣标郭建江周晓迪
Owner 宁波爱基因科技有限公司