Preparation method for aseptic explant of virus-free seedcane of sugarcane

Abstract

The invention discloses a preparation method for an aseptic explant of a virus-free seedcane of sugarcane. The preparation method comprises the following steps: cutting a plant which grows robustly and does not have diseases and insect pests into single-bud sections; planting the single-bud sections in an aseptic provenance garden after the single-bud sections are soaked for 2 hours in a constant temperature field of 52 DEG C; after 5 to 7 months, taking the tips of pathogen-free plant sugarcane after more than or equal to 3 consecutive sunny days, and cutting the tips of the stems to serve as an aseptic explant by adopting an aseptic operation. The biggest difference between the preparation method and the conventional external building method is that the mercury chloride sterilization step is omitted. The preparation method is ecological, environmentally friendly, simple and feasible, and provides a good aseptic explant material taking method for the scale production of the virus-free seedcanes of the sugarcanes.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of healthy sugarcane seedlings, and in particular relates to a method for preparing sterile explants of healthy sugarcane seedlings. Background technique [0002] Sugarcane virus-free healthy seedlings generally refer to sugarcane seedlings that do not carry the pathogens of sugarcane perennial dwarf disease and sugarcane mosaic disease (hereinafter referred to as pathogens) obtained by means of biotechnology. The conventional technical process is as follows: pathogen removal →Establishment of sterile explants→Induction of clustered buds→Pathogen detection→Proliferation and rapid propagation of healthy seedlings→Rooting culture→Pseudo-planting on sand beds in the first-level nursery→Pseudo-planting in nutrient cups in the second-level nursery→Field expansion of first-class seedlings in field Propagation→Pathogen detection→Qualified seedling field expansion of second-level seedling field→...

AI Technical Summary

Problems solved by technology

The inventor thinks that above-mentioned two kinds of explant preparation methods have following defect through practice for many years: the progeny seedling that the heart leaf callus culture of the first method is easy to produce variation, is unfavorable for the stable quality of seedling; Methods The shoot tips with a size of 1-2mm were used as explants, which was difficult to obtain and required high technical requirements, and the subsequent induction culture was prone to disinfectant damage and phenolic browning death, and the materials and induction culture were successful. At the same time, both methods use mercuric chloride to disinfect explants. If the operation process is not handled properly, there will be potential adverse effects on the health of operators and the ecological environment.

Images