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Bordetella pertussis culture medium

A pertussis and culture medium technology, applied in the field of pertussis bacillus culture medium, can solve the problems of infected animals carrying viruses, complex components, unsafe vaccines, etc., and achieve the effects of reducing side reactions, improving culture efficiency, and improving safety.

Inactive Publication Date: 2016-06-01
蓝海军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These animal blood components and animal-derived nitrogen source components added to the medium all have the potential risk of infected animals carrying viruses, and the components are complex, which brings unsafe risks to vaccines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A pertussis bacilli culture medium is composed of the following raw materials: 1000ml water, 5g sodium glutamate, 4g plant peptone, 1g sodium chloride, 0.1g magnesium sulfate, 2gL-cystine, 0.2g niacin, 0.5g ascorbic acid, 5g reduced glutathione, 0.5g FeSO4·7H2O, 20g aloe vera gel, 25g agar, 4g olive oil, 5g barley extract.

Embodiment 2

[0019] A culture medium for pertussis bacilli, consisting of the following raw materials: 1000ml water, 8g sodium glutamate, 8g plant peptone, 2g sodium chloride, 0.2g magnesium sulfate, 3g L-cystine, 0.3g niacin, 1g ascorbic acid, 10g Reduced glutathione, 1.0g FeSO4·7H2O, 40g aloe vera gel, 35g agar, 5g olive oil, 10g barley extract.

Embodiment 3

[0021] A pertussis bacillus culture medium, consisting of the following raw materials: 1000ml water, 6g sodium glutamate, 6g plant peptone, 1g sodium chloride, 0.2g magnesium sulfate, 2g L-cystine, 0.3g niacin, 1g ascorbic acid, 8g Reduced glutathione, 1.0g FeSO4·7H2O, 30g aloe vera gel, 30g agar, 4g olive oil, 10g barley extract.

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PUM

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Abstract

The invention belongs to the technical field of bacterium culture media, and relates to a bordetella pertussis culture medium. The bordetella pertussis culture medium is prepared from 1000 ml of water, 5-8 g of sodium glutamate, 4-8 g of phytone, 1-2 g of sodium chloride, 0.1-0.2 g of magnesium sulfate, 2-3 g of L-cystine, 0.2-0.3 g of niacin, 0.5-1 g of ascorbic acid, 5-10 g of reduced glutathione, 0.5-1.0 g of FeSO4.7H2O, 20-40 g of aloe vera gel, 25-35 g of agar, 4-5 g of olive oil and 5-10 g of highland barley extract. By using aloe vera gel, adopting a plant nitrogen source and adding olive oil and ascorbic acid to the culture medium, the stability of the culture medium is improved; by adding highland barley extract as the reproduction nutrient of the bordetella pertussis culture medium, the culture efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of bacterial culture medium and relates to a culture medium for bacillus pertussis. Background technique [0002] Whooping cough is an important cause of infant mortality worldwide. According to WHO reports, there were about 16 million cases of whooping cough in the world in 2008, 95% of which occurred in developing countries, and about 195,000 children died of it. In my country, it can be seen from the national notifiable infectious disease epidemic notifications in recent years that about 2,000 people develop the disease every year. [0003] Pertussis is caused by droplet transmission of the bacterium Bordetella pertussis from an infected person to a susceptible individual. For decades, immunization of infants and young children with pertussis vaccines of high quality has successfully prevented severe cases of pertussis in infants and young children worldwide. In 2008, about 82% of infants and young chi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/02C12R1/01
CPCC12N1/20C12P21/02
Inventor 何美娟蓝海军
Owner 蓝海军
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