Multiplex amplification system and detection kit for short tandem repeat sequence of mouse

Abstract

The invention relates to a multiplex amplification system and a detection kit for a short tandem repeat sequence of a mouse, and belongs to the technical field of biological detection. According to the multiplex amplification system and the detection kit for the short tandem repeat sequence of the mouse, nine STR (Short Tandem Repeat) loci, which have high sensitivity and amplification specificity, of the mouse are selected; the STR loci are amplified once; the loci amplified by the multiplex amplification system are some STR loci which can be used for identifying a cell line of the mouse; when the cell line of the mouse is identified, the multiplex amplification system and the detection kit have the advantages of being quick, being simple to operate and accurate in result, being convenient for large-scale popularization, and the like. By using a method, along with the successful development of the kit, the batch production can be realized; a working condition can be modeled; automatic equipment exists in all processes; human operation experience is not depended on any more; the automation can be realized; a required time is short; a whole detection time needs approximate 6 hours.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a compound amplification system for identifying multiple short tandem repeat sequences in mice and a kit thereof, which can rapidly and accurately perform STR typing of mouse cells and further identify mouse cell line types. Background technique [0002] In 2009, an editorial in Nature pointed out: Among the thousands of biological laboratories using cell lines, many laboratories do not know that between 1 / 5-1 / 3 of the commonly used cell lines may have been It's not what it used to be. Numerous studies over the past 25 years, combined with experience with cell banks in the US, UK, Germany, and Japan have found that 18-36% of cultures contain misidentified species or other cell types. [0003] In May 2010, the ATCCSDO (Standards Development Organization) working group published a forward-looking report entitled "Cellline misidentification: the beginning of the end" in the journal Natur...

AI Technical Summary

Problems solved by technology

However, the STR sites used in this patent are all 2nt. repeats, resulting in a stutter peak height, which is not conducive to judging cross-contamination between mouse cells; and only mouse STR sites, unable to judge human and mouse cell cross-contamination

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