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In vitro preparation method of avian infectious laryngotracheitis virus resisting specific transfer factor

A transfer factor, tracheitis technology, applied in antiviral agents, allergic diseases, medical preparations containing active ingredients, etc. Immunity, good effect

Inactive Publication Date: 2016-06-15
TIANJIN HLINTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of preparation in the existing transfer factor technology, lack of specificity for diseases, unstable therapeutic effect, etc., and provide an in vitro immunization method with strong specificity, low cost, high output rate and better effect. Preparation method of specific transfer factor against chicken infectious laryngotracheitis virus

Method used

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  • In vitro preparation method of avian infectious laryngotracheitis virus resisting specific transfer factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] An in vitro preparation method of anti-avian infectious laryngotracheitis virus specific transfer factor, the preparation method steps are as follows:

[0023] (1) Take chicken spleen or chicken blood in a sterile environment, and prepare lymphocyte monolayer from chicken spleen or peripheral blood. Take 30 ml of animal whole blood in a sterile vein, add 0.3 ml of 0.8% heparin solution for anticoagulation, and let it stand for 60 minutes. Use a pipette with a rubber bulb to suck all the plasma above the red blood cell layer, especially the white blood cell layer immediately above the red blood cell layer. Dispense into sterile centrifuge tubes, wash 3 times with PBS (pH 7.2) solution, centrifuge at 1200 rpm, then use 40ml of 30% calf serum hydrolyzed milk protein for white blood cell culture, mix well, and distribute into volume In a 100ml cell culture flask, stopper the bottle tightly and place it in a 37°C cell culture incubator. After 3 days, the white blood cells will ...

Embodiment 2

[0027] An in vitro preparation method of anti-avian infectious laryngotracheitis virus specific transfer factor, the preparation method steps are as follows:

[0028] (1) Take chicken spleen or chicken blood in a sterile environment, and prepare lymphocyte monolayer from chicken spleen or peripheral blood. Take 20ml of animal whole blood in a sterile vein, add 0.3ml of 0.8% heparin solution for anticoagulation, and let it stand for 30 minutes. Use a pipette with a rubber bulb to suck all the plasma above the red blood cell layer, especially the white blood cell layer immediately above the red blood cell layer. Dispense into sterile centrifuge tubes, wash 2-3 times with PBS (pH 7.2) solution, centrifugation speed 800 rpm, then use 40ml of 30% calf serum hydrolyzed milk protein for white blood cell culture, mix evenly and then aliquot In a cell culture flask with a capacity of 100ml, stopper the flask tightly and place it in a cell culture incubator at 37°C. After 2 days, the leuko...

Embodiment 3

[0032] The detection of the anti-avian infectious laryngotracheitis virus-specific transfer factor prepared by the process of the present invention is as follows: The anti-avian infectious laryngotracheitis virus transfer factor prepared by the process of Example 1 (hereinafter referred to as "this product") Detection:

[0033] (1) The standard solution is a yellow and clear liquid with a pH between 6.1-7.0.

[0034] (2) Determination of peptide content: The peptide content of this product is 0.51mg / ml by the double-shrinking method.

[0035] (3) Determination of nucleic acid content: The nucleic acid content of this product determined by the lichenol method is 0.34mg / ml.

[0036] (4) Bacteriological testing: There is no need for oxygen, anaerobic, saprophytic bacteria and fungi in this product.

[0037] (5) Take 10 healthy mice, orally take the concentrated solution of this product, which is equivalent to 20 times the dose of normal oral solution, and observe the changes in the surviv...

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Abstract

The invention relates to an in vitro preparation method of an avian infectious laryngotracheitis virus resisting specific transfer factor. The in vitro production of the avian infectious laryngotracheitis virus resisting specific transfer factor is achieved by preparing a lymphocyte monolayer from chicken spleen or peripheral blood, and conducting induced culture to human lymphocytes by virtue of phytohemagglutinin and avian infectious laryngotracheitis viruses. The specific transfer factor, which is prepared by the preparation method disclosed by the invention, can be used for preventing and treating avian infectious laryngotracheitis, and meanwhile, the specific transfer factor can be used for enhancing chicken immunity. The invention, as a relatively ideal avian infectious laryngotracheitis virus resisting specific transfer factor preparation method, has the advantages of strong specificity, low in cost, high yield, good effect and the like.

Description

Technical field [0001] The invention relates to the field of veterinary medicine, in particular to an in vitro preparation method of a specific transfer factor for anti-avian infectious laryngotracheitis virus. Background technique [0002] Infectious laryngotracheitis (ILT) is an acute contact respiratory infection of chickens caused by the infectious laryngotracheitis virus (ILTV). Chicken infectious laryngotracheitis can be divided into laryngotracheitis type and ocular type according to the symptoms, and the disease of chicks is mostly ocular type. The incidence of the disease is close to 100%, the mortality rate is 50% to 70%, and the egg production of laying hens is significantly reduced. It has caused huge losses to the poultry industry and is one of the important diseases of intensive chicken farms. [0003] Infectious laryngotracheitis mainly occurs in autumn and winter and early spring. It mostly occurs in adult chickens, and the cause is extremely complicated. Sick ch...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K35/14A61P31/22A61P37/04
Inventor 陈庆忠安同伟刘芳
Owner TIANJIN HLINTE BIOTECH CO LTD
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