Preparation method of avian infectious laryngotracheitis virus resisting specific transfer factor

A technology of transfer factor and tracheitis, which is applied in the direction of antiviral agents, medical preparations containing active ingredients, antibacterial drugs, etc., can solve the problems of unstable treatment effect and non-specificity of diseases, so as to improve immunity and specificity Strong, good effect

Inactive Publication Date: 2016-04-20
TIANJIN HLINTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of preparation in the existing transfer factor technology, lack of specificity for diseases, unstable therapeutic effect, etc., and provide an in vitro immunization method with strong specificity, low cost, high output rate and better effect. Preparation method of specific transfer factor against chicken infectious laryngotracheitis virus

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  • Preparation method of avian infectious laryngotracheitis virus resisting specific transfer factor

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Embodiment 1

[0024] A preparation method for in vitro preparation of specific transfer factor against chicken infectious laryngotracheitis virus:

[0025] (1) Chicken spleen or chicken blood was collected under a sterile environment, and a monolayer of lymphocytes was prepared from the chicken spleen or peripheral blood. Take 30ml of animal whole blood through a sterile vein, add 0.8% heparin solution 0.3m1 for anticoagulation, let it stand for 60 minutes, and use a straw with a rubber ball to absorb all the plasma above the red blood cell layer, especially the white blood cell layer immediately above the red blood cell layer. Dispense into sterilized centrifuge tubes, wash with PBS (pH 7.2) solution repeatedly for 3 times, centrifuge at 1200 rpm, then use 40ml of hydrolyzed milk protein containing 30% calf serum for leukocyte culture, mix well and then pack into volume In a 100ml cell culture bottle, tightly cork the bottle and place in a 37°C cell culture incubator. After 3 days, the leu...

Embodiment 2

[0029] A preparation method for in vitro preparation of specific transfer factor against chicken infectious laryngotracheitis virus:

[0030] (1) Chicken spleen or chicken blood was collected under a sterile environment, and a monolayer of lymphocytes was prepared from the chicken spleen or peripheral blood. Take 20ml of animal whole blood through a sterile vein, add 0.8% heparin solution 0.3m1 for anticoagulation, let it stand for 30 minutes, and use a straw with a rubber ball to absorb all the plasma above the red blood cell layer, especially the white blood cell layer immediately above the red blood cell layer. Dispense into sterilized centrifuge tubes, wash repeatedly 2-3 times with PBS (pH 7.2) solution, centrifuge at 800 rpm, then use 40ml of hydrolyzed milk protein containing 30% calf serum for white blood cell culture, mix well and then pack Place in a 100ml cell culture bottle, stopper tightly, and place in a cell culture incubator at 37°C. After 2 days, the leukocyte...

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Abstract

The invention relates to a preparation method of an avian infectious laryngotracheitis virus resisting specific transfer factor. The avian infectious laryngotracheitis virus resisting specific transfer factor is prepared by the following steps: preparing a lymphocyte monolayer from chicken spleen or peripheral blood; then, inducing and culturing lymphocytes by virtue of phytohemagglutinin and avian infectious laryngotracheitis viruses; and promoting in vitro, so that the avian infectious laryngotracheitis virus resisting specific transfer factor is obtained. The specific transfer factor prepared by the invention can be used for preventing and treating avian infectious laryngotracheitis, and meanwhile the transfer factor can be used for enhancing the immunity of chickens. The preparation method of the avian infectious laryngotracheitis virus resisting specific transfer factor, which is relatively ideal, has the advantages of being strong in specificity, low in cost, high in yield, good in effect and the like.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, in particular to a preparation method of specific transfer factor against chicken infectious laryngotracheitis virus. Background technique [0002] Infectious laryngotracheitis (ILT) is an acute contagious respiratory infectious disease of chickens caused by infectious laryngotracheitis virus (ILTV). Chicken infectious laryngotracheitis can be divided into laryngotracheitis type and ocular type according to the symptoms, and the onset of chickens is mostly ocular type. The incidence of the disease is close to 100%, the mortality rate is 50% to 70%, and the egg production of laying hens has decreased significantly. It has caused huge losses to the poultry industry and is one of the important diseases in intensive chicken farms. [0003] Chicken infectious laryngotracheitis is very easy to occur mainly in autumn and winter and early spring, mostly in adult chickens, and the etiology is extremely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/14A61K35/28A61K38/00A61P31/20A61P31/04
Inventor 陈庆忠安同伟刘芳
Owner TIANJIN HLINTE BIOTECH CO LTD
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