Kit and diagnosis method being capable of diagnosing human enterovirus 71 type infection

A technology for diagnostic kits and kits, applied in biological testing, material testing products, instruments, etc., can solve the problems of low efficiency, high cost of virus cultivation, and inability to supply the market in large quantities.

Inactive Publication Date: 2016-06-15
SUZHOU SJ BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The detection of enterovirus 71 antibody (IgM) has been reported in the relevant literature. Basically, the virus culture of enterovirus 71 is used as an indirect ELISA for the coated antigen. There are many deficiencies; and, because the cost of virus cultivation is high and the efficiency is low, it is impossible to supply the market in large quantities
Chinese invention patent CN102243232A, published on November 16, 2011, discloses an enzyme-linked immunoassay kit (enzyme-linked immunoassay) for enterovirus 71 antibody (IgM), and the technical scheme is to first add serum to a microwell plate Among them, the IgM antibody is captured by the anti-μ chain pre-coated on the microwell plate, and then the enzyme label is added, and the EV71-IgM in the captured IgM will specifically bind to the HRP-labeled EV71 recombinant antigen, wash Finally, HRP will react with the subsequent added substrate, and finally achieve the purpose of detecting EV71-IgM antibody, but this method requires a lot (such as the volume of serum), consumes a lot of reagents and other consumables, and this method is limited to For IgM detection, it is not ideal for detecting IgG in patients

Method used

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  • Kit and diagnosis method being capable of diagnosing human enterovirus 71 type infection
  • Kit and diagnosis method being capable of diagnosing human enterovirus 71 type infection

Examples

Experimental program
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Effect test

Embodiment

[0091] 1. Preparation and Confirmation of Peptides

[0092] The polypeptides used in the examples were synthesized by Jill Biochemical (Shanghai) Co., Ltd. and confirmed by mass spectrometry. in,

[0093] SEQ ID NO: 1 (APTGQNTQVSSHRLDTGKVP), molecular weight 2093.

[0094] SEQ ID NO: 2 (SHRLDTGKVPALQAAEIGAS), molecular weight 2020.

[0095] SEQ ID NO: 3 (DKFANPVKDIFTEMAAPLKS), molecular weight 2222.

[0096] SEQ ID NO: 4 (AAAQKNFTMKLCKDASDILQ), molecular weight 2196.

[0097] SEQ ID NO: 5 (LDLPYSTYVKDELRSIDKIK), molecular weight 2397.

[0098] 2. Kit Preparation

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Abstract

The invention provides a kit and a diagnosis method being capable of diagnosing human enterovirus 71 type infection. The kit includes one or more solid carriers, each of which includes a zone I and a zone II which are separated from each other. The zone I comprises a polypeptide collection I which is independently connected to the solid carrier and includes the following polypeptides, independently: a polypeptide represented as the SEQ ID No.1 and a polypeptide represented as the SEQ ID No.2; and the zone II comprises a polypeptide collection II which is independently connected to the solid carrier and includes, independently, the polypeptide represented as the SEQ ID No.1. The kit is used for detecting enterovirus 71 type antibody (IgM and IgG) in human serum, is excellent in accuracy, sensitivity and specificity, is suitable for etiology laboratory auxiliary diagnosis of the enterovirus 71 type, is simple in operation and is suitable for medical treatment and disease control departments at all levels.

Description

technical field [0001] The present invention mainly relates to a diagnostic kit and a diagnostic method. Specifically, the present invention relates to a kit and a diagnostic method that can be used for diagnosing human enterovirus 71 infection. Background technique [0002] Enterovirus EV71 infection is a general term for diseases caused by human enterovirus 71 (EV71). Clinically, it mainly manifests as hand, foot and mouth disease. Hand, foot and mouth disease often occurs in infants and young children. Some children with EV71 infection manifest Herpetic angina, viral encephalitis, viral meningitis, pulmonary edema, pulmonary hemorrhage, etc. in severe patients. The most common pathogens causing hand, foot and mouth disease are Coxsackievirus A16 and enterovirus 71; diseases caused by enterovirus 71 infection have a large proportion of severe infections, and the fatality rate is also high. The rate can reach 10%-25%, so the etiological diagnosis is of great significance....

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569C07K7/08
Inventor 马雄明张慧英滕焕
Owner SUZHOU SJ BIOMATERIALS
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