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Microalgae fermentation liquid exopolysaccharide, its preparation method and application

An extracellular polysaccharide, polysaccharide technology, applied in the preparation of sugar derivatives, microorganism-based methods, biochemical equipment and methods, etc.

Active Publication Date: 2020-11-27
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, foreign countries have little research on the exopolysaccharide produced during the fermentation process of Schizochytrium sp., and only US8232090B2 reports a mutant strain of Schizochytrium sp. MTCC 5249 and the DHA lipid and exopolysaccharide produced by it.
Therefore, according to the current literature reports, the research on the extracellular polysaccharide produced by Schizochytrium S-31 during the fermentation process is still blank.

Method used

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  • Microalgae fermentation liquid exopolysaccharide, its preparation method and application
  • Microalgae fermentation liquid exopolysaccharide, its preparation method and application
  • Microalgae fermentation liquid exopolysaccharide, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Fermented algae Schizochytrium ATCC20888 strain as follows:

[0135] (1) Single colonies were picked after activation, was inoculated into the seed medium at 28 ℃, 200rpm rotational speed shaker for 48 hours to a concentration of about seed dry weight 15-20g / L;

[0136] (2) 10% of inoculum, seed liquid culture medium to 7.5L of the seed tank, at 28 ℃, aeration culture at 600rpm rotational speed of 36 hours, the fermentation broth cell dry weight 24g / L;

[0137] (3) 10% of inoculum, seed culture broth was inoculated into 50L fermenter, the volume of liquid 18L, at 28 ℃, the rotational speed of 300rpm-600rpm, aeration culture 120 hours fermentation process stream was added to the fermentor carbon and nitrogen solution solution.

[0138] Schizochytrium sp take S-31 5 liters broth, was added 20 liters of 60% ethanol solution (v / v), extraction with stirring at 60 deg.] C for 2 hours and the supernatant after centrifugation, the remaining pellet was extracted according to t...

Embodiment 2

[0140] As described in Example 1 Fermentation method Schizochytrium sp S-31.

[0141] Schizochytrium sp take S-31 broth 1 liter, 5 liters of water was added, extraction was stirred at 80 deg.] C for 2 hours and the supernatant after centrifugation, and concentrated under reduced pressure to 1 liter. Extract concentrate was added 300 ml of chloroform for extraction, continuous extraction three times, the aqueous layer was collected portion. After extraction the extract was added 300 ml of chloroform: n-butanol 4: 1 (v / v) mixture deproteinization, 6 times, until no later added to the mixture until the generation of significant protein precipitation was collected aqueous layer portion to remove the residual organic solvent was concentrated under reduced pressure. The dialyzed extract after addition of protein placed in a dialysis bag, 5000D molecular weight cut off dialysis membrane, dialyzed for 30 h, changing the water every 12 hours, stirring occasionally. After completion of th...

Embodiment 3

[0143] As described in Example 1 Fermentation method Schizochytrium sp S-31.

[0144]Schizochytrium sp take S-31 broth 2 liters, was added 6 liters of 40% ethanol solution (v / v), stirred for 2 hours at room temperature to extract the supernatant after centrifugation, the remaining precipitation as described above and then extracted twice, the supernatant after three extractions were combined and concentrated to 2 liters under reduced pressure. Extracting the concentrate was added 700 ml of n-hexane was extracted three times with continuous extraction, the aqueous layer was collected, concentrated under reduced pressure to remove remaining organic solvents. After extraction the extract was added 500 ml of chloroform: n-butanol 4: 1 (v / v) mixture deproteinization, 5 times, until the mixture was added until the apparent protein produced no precipitate was collected aqueous layer portion to remove the residual organic solvent was concentrated under reduced pressure. The dialyzed e...

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Abstract

The invention relates to microalgae broth exopolysaccharide and its preparation method and use. The invention provides an exopolysaccharide composition. The exopolysaccharide composition comprises, by mole, 73.00-85.00% of galactose, 5.50-8.50% of glucose, 2.50-8.00% of mannose, 2.00-6.00% of rhamnose, 1.50-3.80% of fucose and 1.20-2.50% of xylose. The invention also relates to a preparation method of the exopolysaccharide composition and the exopolysaccharide composition. The exopolysaccharide composition has substantial antioxidation activity and can be used for food oxidation resistance and fruit and vegetable fresh-keeping. The invention also relates to an anti-oxidant containing the exopolysaccharide composition and a fresh-keeping agent containing the exopolysaccharide composition and a use of the exopolysaccharide composition in oxidation resistance and fruit and vegetable fresh-keeping.

Description

Technical field [0001] The present invention relates to a polysaccharide extracted from algae extracellular fermentation broth, preparation and use thereof. Background technique [0002] (Schizochytrium limacinum) Schizochytrium sp is a class of marine fungi belonging to Oomycetes fungi gate network sticky Thraustochytrium Division, it is a globular unicellular fungi. Thraustochytrium spoilage microorganisms belonging addicted, widely present in the organic-rich and high-salinity environments, such fungal growth speed fast, strong resistance, easy to mass culture, because such cells can be contained in the proportion of DHA dry cell weight reached about 20%, and therefore is ideal for industrial culture as a source of DHA species. While such microalgae fermentation of DHA, secrete large amounts of polysaccharides, polysaccharide or polysaccharide complexes such present outside the cell wall, the cells easily separated, it is called exopolysaccharide (EPS). These extracellular pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L3/3562A23B7/154C12P19/02C07H3/02C07H1/06C12R1/645
Inventor 张戈徐志村邹彦平常桂芳
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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