Microalgae fermentation liquid exopolysaccharide, its preparation method and application
An extracellular polysaccharide, polysaccharide technology, applied in the preparation of sugar derivatives, microorganism-based methods, biochemical equipment and methods, etc.
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Embodiment 1
[0134] Fermented algae Schizochytrium ATCC20888 strain as follows:
[0135] (1) Single colonies were picked after activation, was inoculated into the seed medium at 28 ℃, 200rpm rotational speed shaker for 48 hours to a concentration of about seed dry weight 15-20g / L;
[0136] (2) 10% of inoculum, seed liquid culture medium to 7.5L of the seed tank, at 28 ℃, aeration culture at 600rpm rotational speed of 36 hours, the fermentation broth cell dry weight 24g / L;
[0137] (3) 10% of inoculum, seed culture broth was inoculated into 50L fermenter, the volume of liquid 18L, at 28 ℃, the rotational speed of 300rpm-600rpm, aeration culture 120 hours fermentation process stream was added to the fermentor carbon and nitrogen solution solution.
[0138] Schizochytrium sp take S-31 5 liters broth, was added 20 liters of 60% ethanol solution (v / v), extraction with stirring at 60 deg.] C for 2 hours and the supernatant after centrifugation, the remaining pellet was extracted according to t...
Embodiment 2
[0140] As described in Example 1 Fermentation method Schizochytrium sp S-31.
[0141] Schizochytrium sp take S-31 broth 1 liter, 5 liters of water was added, extraction was stirred at 80 deg.] C for 2 hours and the supernatant after centrifugation, and concentrated under reduced pressure to 1 liter. Extract concentrate was added 300 ml of chloroform for extraction, continuous extraction three times, the aqueous layer was collected portion. After extraction the extract was added 300 ml of chloroform: n-butanol 4: 1 (v / v) mixture deproteinization, 6 times, until no later added to the mixture until the generation of significant protein precipitation was collected aqueous layer portion to remove the residual organic solvent was concentrated under reduced pressure. The dialyzed extract after addition of protein placed in a dialysis bag, 5000D molecular weight cut off dialysis membrane, dialyzed for 30 h, changing the water every 12 hours, stirring occasionally. After completion of th...
Embodiment 3
[0143] As described in Example 1 Fermentation method Schizochytrium sp S-31.
[0144]Schizochytrium sp take S-31 broth 2 liters, was added 6 liters of 40% ethanol solution (v / v), stirred for 2 hours at room temperature to extract the supernatant after centrifugation, the remaining precipitation as described above and then extracted twice, the supernatant after three extractions were combined and concentrated to 2 liters under reduced pressure. Extracting the concentrate was added 700 ml of n-hexane was extracted three times with continuous extraction, the aqueous layer was collected, concentrated under reduced pressure to remove remaining organic solvents. After extraction the extract was added 500 ml of chloroform: n-butanol 4: 1 (v / v) mixture deproteinization, 5 times, until the mixture was added until the apparent protein produced no precipitate was collected aqueous layer portion to remove the residual organic solvent was concentrated under reduced pressure. The dialyzed e...
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