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Microalgae broth exopolysaccharide and its preparation method and use

A technology of extracellular polysaccharides and polysaccharides, applied in the direction of preparation of sugar derivatives, microbial-based methods, biochemical equipment and methods, etc.

Active Publication Date: 2016-06-22
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, foreign countries have little research on the exopolysaccharide produced during the fermentation process of Schizochytrium sp., only see the report of a Schizochytrium mutant strain MTCC5249 and the DHA lipid and exopolysaccharide produced by it in US8232090B2
Therefore, according to the current literature reports, the research on the extracellular polysaccharide produced by Schizochytrium S-31 during the fermentation process is still blank.

Method used

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  • Microalgae broth exopolysaccharide and its preparation method and use
  • Microalgae broth exopolysaccharide and its preparation method and use
  • Microalgae broth exopolysaccharide and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Schizochytrium ATCC20888 strain was fermented as follows:

[0135] (1) Select the activated single colony, inoculate it into the seed liquid culture medium, and cultivate it on a shaking table at 28°C and 200rpm for 48 hours until the concentration of the seed liquid is about 15-20g / L dry weight;

[0136] (2) According to the inoculum size of 10%, the seed solution was received in 7.5L seed tank culture medium, at 28 DEG C, aerated at 600 rpm for 36 hours, and the dry weight of cells in the fermentation liquid reached 24g / L;

[0137] (3) According to the inoculum amount of 10%, inoculate the seed liquid into 50L fermenter culture medium, the liquid filling capacity is 18L, at 28 ℃, under the rotating speed of 300rpm-600rpm, ventilate and cultivate for 120 hours, feed the fermenter during the fermentation process Carbon source solution and nitrogen source solution.

[0138] Take 5 liters of Schizochytrium S-31 fermentation broth, add 20 liters of 60% ethanol solution (v...

Embodiment 2

[0140] Schizochytrium S-31 was fermented according to the method described in Example 1.

[0141] Take 1 liter of Schizochytrium S-31 fermentation broth, add 5 liters of water, stir and extract at 80°C for 2 hours, centrifuge, take the supernatant, and concentrate it to 1 liter under reduced pressure. Add 300 milliliters of chloroform to the extraction concentrate for extraction, extract continuously for 3 times, and collect the water layer. Add 300 milliliters of chloroform to the extract after extraction: n-butanol 4:1 (v / v) mixture to remove protein, 6 times in a row, until no obvious protein precipitation occurs after adding the mixture, collect the water layer part , concentrated under reduced pressure to remove residual organic solvent. The protein-removed extract was put into a dialysis bag for dialysis. The molecular weight cut-off of the dialysis membrane was 5000D, and the dialysis time was 30 hours. The water was changed once every 12 hours and stirred from time to...

Embodiment 3

[0143] Schizochytrium S-31 was fermented according to the method described in Example 1.

[0144]Take 2 liters of Schizochytrium S-31 fermentation broth, add 6 liters of 40% ethanol solution (v / v), stir and extract at room temperature for 2 hours, take the supernatant after centrifugation, and extract the remaining precipitate twice according to the above method, The supernatants after three extractions were combined and concentrated to 2 L under reduced pressure. Add 700 milliliters of n-hexane to the extraction concentrate for extraction, extract continuously for 3 times, collect the water layer, concentrate under reduced pressure to remove the residual organic solvent. Add 500 milliliters of chloroform to the extract after the extraction: n-butanol 4:1 (v / v) mixture to remove protein, continue for 5 times, until no obvious protein precipitation occurs after adding the mixture, collect the water layer part , concentrated under reduced pressure to remove residual organic sol...

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Abstract

The invention relates to microalgae broth exopolysaccharide and its preparation method and use. The invention provides an exopolysaccharide composition. The exopolysaccharide composition comprises, by mole, 73.00-85.00% of galactose, 5.50-8.50% of glucose, 2.50-8.00% of mannose, 2.00-6.00% of rhamnose, 1.50-3.80% of fucose and 1.20-2.50% of xylose. The invention also relates to a preparation method of the exopolysaccharide composition and the exopolysaccharide composition. The exopolysaccharide composition has substantial antioxidation activity and can be used for food oxidation resistance and fruit and vegetable fresh-keeping. The invention also relates to an anti-oxidant containing the exopolysaccharide composition and a fresh-keeping agent containing the exopolysaccharide composition and a use of the exopolysaccharide composition in oxidation resistance and fruit and vegetable fresh-keeping.

Description

technical field [0001] The invention relates to exopolysaccharide extracted from microalgae fermentation liquid, its preparation method and application. Background technique [0002] Schizochytrium limacinum is a kind of marine fungus, which belongs to the fungal phylum Reticulomycetes class Thraustochytriaceae, and is a single-celled spherical fungus. Thraustochytrium belongs to putrefactive microorganisms, which widely exist in the environment with rich organic matter and high salinity. This kind of fungus reproduces quickly, has strong stress resistance, and is easy to cultivate on a large scale. Because the proportion of DHA contained in this kind of cells can be It reaches about 20% of the dry weight of the cells, so it is an ideal species for industrialized cultivation as a source of DHA. This type of microalgae will secrete a large amount of polysaccharides while fermenting to produce DHA. This type of polysaccharide or polysaccharide complex exists outside the cell ...

Claims

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Application Information

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IPC IPC(8): A23L3/3562A23B7/154C12P19/02C07H3/02C07H1/06C12R1/645
Inventor 张戈徐志村邹彦平常桂芳
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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