Method for preparing grifola frondosa protein peptide-selenium chelate

A technology of grifola frondosa and chelate, which is applied in the field of preparation of grifola frondosa protein peptide-selenium chelate, can solve the problems of small lethal dose, toxic and side effects, and low biological effectiveness, and achieve easy absorption, protein The effect of high extraction rate and low price

Inactive Publication Date: 2016-06-22
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1: Superfinely crush Grifola frondosa, mix the crushed Grifola frondosa with distilled water at a ratio of 1:40w / v, use 2.0wt.% NaOH to adjust the pH to 9.0, ultrasonically extract for 20 minutes, and extract in a water bath for 60 minutes. The temperature is 50°C. After extraction, filter with gauze. The filtrate is the alkali-extracted grifola frondosa protein solution. Use hydrochloric acid to adjust the pH of the alkali-extracted grifola frondosa protein solution to 3.0 for protein precipitation. Centrifuge at 3500r / min for 5 minutes to obtain the precipitated protein, and wash with water until Neutral centrifuge again, freeze-dry the precipitate to obtain the grifolain, and its extraction rate reaches 70.25%.

[0020] Step 2: Dissolve the grifola frondosa protein in water so that the protein concentration reaches 3.0%, adjust the pH of the grifola frondosa protein solution to 11.0 with NaOH solution, keep the temperature at 55°C, add 3.0wt.% protease, and hydro...

Embodiment 2

[0030] Step 1: Ultrafinely crush Grifola frondosa, mix the crushed Grifola frondosa with distilled water at a ratio of w / v1:60, use 1.0% NaOH to adjust the pH to 11.0, ultrasonic extraction for 50 minutes, water bath extraction for 120 minutes, temperature 100 ℃, filter with gauze after extraction, the filtrate is alkali-extracted grifola frondosa protein solution, adjust the pH of the alkali-extracted grifola frondosa protein solution to 4.0 with hydrochloric acid for protein precipitation, centrifuge to obtain precipitated protein, wash with water until neutral and centrifuge again, and The precipitation is freeze-dried to obtain the grifolain, and the extraction rate reaches 82.75%.

[0031] Step 2: Dissolve the Grifola frondosa protein in water so that the protein concentration reaches 1.0%, adjust the pH to 9.0, keep the temperature at 40°C, add 1.0% protease, hydrolyze for 40 minutes, and inactivate the enzyme to obtain the Grifola frondosa protein polypeptide complex so...

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Abstract

The present invention provides a preparation method of Grifola frondosa protein peptide-selenium chelate, comprising: extracting the protein in Grifola frondosa frondosa by alkali-dissolving acid precipitation method or ammonium sulfate precipitation method; using alkaline protease, neutral protease or compound Grifola frondosa protein enzymatic hydrolyzate is prepared by protease after restriction enzymolysis, and the enzyme is inactivated; the selenium in the inorganic substance sodium selenite is used to chelate the grifola frondosa protein peptide-selenium Chelates. The invention can significantly improve the extraction rate of grifola frondosa protein by alkali-dissolving and acid-precipitating method; the degradation degree of protein can be effectively controlled by controlling the enzymatic hydrolysis time; the preparation process of polypeptide-selenium chelate is simple; the mineral element-peptide prepared by the invention Chelates have a unique chelation system and transport mechanism, are easily absorbed, safe and non-toxic, low in price, and can supplement amino acids and mineral ions at the same time, and will definitely become the first choice for mineral element supplements; the present invention is the application of Grifola frondosa Provided a new way of thinking.

Description

technical field [0001] The invention relates to a preparation method of a grifolain peptide-selenium chelate, which belongs to the field of food biotechnology. Background technique [0002] Selenium (Se) is an important mineral element necessary for the human body and has important physiological functions. A large number of existing studies have shown that many diseases are closely related to the nutritional status of Se. The human body mainly obtains selenium through diet, and the method of artificial selenium supplementation is oral selenium-containing preparations. The general method of supplementing selenium is to make oral preparations with inorganic selenium compounds (sodium selenite and sodium selenate). However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited. Studies have shown that organic selenium compounds after inorgan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/30
CPCC12P21/06C07K1/30
Inventor 赵立娜刘斌陈濠陈紫红范锦琳林占熺吕旭聪李鑫
Owner FUJIAN AGRI & FORESTRY UNIV
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