Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property

A technology of scallop gonads and gonads, which is applied in the field of preparation of highly gelatinous scallop gonad enzymatic hydrolyzate, can solve the problems of low gelation of the enzymatic hydrolyzate, achieve simple operation process, improve gel properties, and improve coagulation Colloidal and nutritional effects

Inactive Publication Date: 2016-06-29
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the characteristics of high protein content in the gonads of male scallops, a method of using the gonads of male scallops to prepare an enzymatic hydrolyzate with high gel perfor

Method used

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  • Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property
  • Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property
  • Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property

Examples

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Example Embodiment

[0036] Example 1

[0037] 1. Take fresh male scallop gonads, rinse them with deionized water, heat in a boiling water bath for 10 minutes, cool to room temperature, freeze-dry, and crush to obtain freeze-dried powder of male scallops gonads;

[0038] 2. Weigh 2.3g of freeze-dried powder (the protein content of freeze-dried powder is determined by Kjeldahl method, accounting for 87% of the dry basis of freeze-dried powder, and the protein is 2g), add 50ml deionized water, mix well, and boil water bath Heat in medium for 10 minutes, cool to 37°C, adjust the pH of the mixture to 8.0 with 0.5mol / L NaOH solution;

[0039] 3. Add 6000U trypsin to the mixed solution (the amount of enzyme is 3000U / g protein), stir for enzymolysis, and keep the pH at 8.0 with 0.5mol / L NaOH solution, and the enzymolysis time is 3h to obtain the enzymatic hydrolysate of scallop gonads , Heat the resulting enzymatic hydrolysate in a boiling water bath for 10 minutes and cool;

[0040] 4. Freeze-dry and pulverize...

Example Embodiment

[0042] Example 2

[0043] 1. Take fresh male scallop gonads, rinse them with deionized water, heat in a boiling water bath for 15 minutes, cool to room temperature, freeze-dry, and crush to obtain freeze-dried powder of male scallops gonads;

[0044] 2. Weigh 4.6g of freeze-dried powder (the protein content in freeze-dried powder is determined by Kjeldahl method, accounting for 87% of the dry basis of freeze-dried powder, and the protein is 4g), add 100ml of deionized water, mix well, and boil water bath Heat in medium for 10 minutes, cool to 37°C, adjust the pH of the mixture to 8.0 with 0.8mol / L NaOH solution;

[0045] 3. Add 12000U trypsin to the mixed solution (the amount of enzyme is 3000U / g protein, stir for enzymolysis, keep the pH at 8.0 with 0.8mol / L NaOH, and the enzymolysis time is 3h to obtain the enzymatic hydrolysate of the gonad The obtained enzymatic hydrolysate is heated in a boiling water bath for 10 minutes and cooled;

[0046] 4. Freeze-dry and pulverize the obtai...

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Abstract

The invention discloses a method for preparing a patinopecten yesoensis gonad enzymatic hydrolysate with the high gelling property. The method comprises the steps of raw material pretreatment, an enzymatic hydrolysis reaction, preparation of patinopecten yesoensis gonad enzymatic hydrolysate gel and the like. According to the method, the patinopecten yesoensis gonad is enzymaticcally hydrolyzed with trypsin, the gelling property of the enzymatic hydrolysate is improved through the methods of performing freeze-drying and adding saline ions, the advantages of being simple in method and good in effect are achieved, the gelling property of the patinopecten yesoensis gonad enzymatic hydrolysate is effectively improved, and the important significance on utilization of patinopecten yesoensis raw materials is achieved.

Description

technical field [0001] The invention relates to a method for preparing a highly gelatinous enzymatic hydrolyzate, more specifically, a method for preparing a highly gelatinous scallop gonad enzymaticallyzate. Background technique [0002] In recent years, with the continuous increase of scallop production, the processing volume of scallops has been increasing year by year. The gonads of scallops contain a large amount of protein components, and the protein content in the male gonads can reach about 87% (by dry weight), which is a good source for developing polypeptides. Contents of the invention [0003] The purpose of the present invention is to develop a method for reprocessing the gonads of the male scallop, improve the utilization rate and added value of the gonads of the scallop, and avoid waste of resources. According to the characteristics of high protein content in the gonads of male scallops, a method of using the gonads of male scallops to prepare an enzymatic h...

Claims

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Application Information

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IPC IPC(8): A23J1/04
CPCA23J1/04
Inventor 吴海涛张萌李毅于翠平孙娜唐越阎佳楠查越刘峻屹
Owner DALIAN POLYTECHNIC UNIVERSITY
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