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Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property

A technology of scallop gonads and gonads, which is applied in the field of preparation of highly gelatinous scallop gonad enzymatic hydrolyzate, can solve the problems of low gelation of the enzymatic hydrolyzate, achieve simple operation process, improve gel properties, and improve coagulation Colloidal and nutritional effects

Inactive Publication Date: 2016-06-29
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the characteristics of high protein content in the gonads of male scallops, a method of using the gonads of male scallops to prepare an enzymatic hydrolyzate with high gel performance was developed to overcome the problem in the prior art that the gonads of male scallops were enzymatically hydrolyzed by protease The problem of low gelation of the obtained hydrolyzate

Method used

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  • Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property
  • Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property
  • Method for preparing patinopecten yesoensis gonad enzymatic hydrolysate with high gelling property

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Embodiment 1

[0037] 1. Take fresh male scallop gonads, rinse them with deionized water, heat in a boiling water bath for 10 minutes, cool to room temperature, freeze-dry, and crush to obtain lyophilized powder of male scallop gonads;

[0038] 2. Weigh 2.3g of freeze-dried powder (the protein content in the freeze-dried powder is determined by the Kjeldahl method, accounting for 87% of the dry basis of the freeze-dried powder, and the protein is 2g), add 50ml of deionized water, mix well, and place in a boiling water bath Heat in medium for 10min, cool to 37°C, adjust the pH of the mixture to 8.0 with 0.5mol / L NaOH solution;

[0039] 3. Add 6000U trypsin (3000U / g protein) to the mixture, stir for enzymolysis, keep the pH at 8.0 with 0.5mol / L NaOH solution, and enzymolysis time is 3h, to obtain the enzymatic hydrolyzate of scallop gonad , heating the obtained enzymolyzate in a boiling water bath for 10 minutes, and cooling;

[0040] 4. Freeze-drying and pulverizing the obtained enzymatic hy...

Embodiment 2

[0043] 1. Take the gonads of fresh male scallops, rinse them with deionized water, heat them in a boiling water bath for 15 minutes, cool to room temperature, freeze-dry, and pulverize them to obtain the lyophilized powder of gonads of male scallops;

[0044] 2. Weigh 4.6g of freeze-dried powder (the protein content in the freeze-dried powder is determined by the Kjeldahl method, accounting for 87% of the dry basis of the freeze-dried powder, and the protein is 4g), add 100ml of deionized water, mix well, and place in a boiling water bath Heat in medium for 10min, cool to 37°C, adjust the pH of the mixture to 8.0 with 0.8mol / L NaOH solution;

[0045] 3. Add 12000U trypsin to the mixed solution (enzyme dosage is 3000U / g protein, stir for enzymolysis, keep the pH at 8.0 with 0.8mol / LNaOH, and enzymolysis time is 3h to obtain the scallop gonad enzymatic hydrolyzate. The obtained enzymolyzate was heated in a boiling water bath for 10 minutes, and then cooled;

[0046] 4. Freeze-d...

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Abstract

The invention discloses a method for preparing a patinopecten yesoensis gonad enzymatic hydrolysate with the high gelling property. The method comprises the steps of raw material pretreatment, an enzymatic hydrolysis reaction, preparation of patinopecten yesoensis gonad enzymatic hydrolysate gel and the like. According to the method, the patinopecten yesoensis gonad is enzymaticcally hydrolyzed with trypsin, the gelling property of the enzymatic hydrolysate is improved through the methods of performing freeze-drying and adding saline ions, the advantages of being simple in method and good in effect are achieved, the gelling property of the patinopecten yesoensis gonad enzymatic hydrolysate is effectively improved, and the important significance on utilization of patinopecten yesoensis raw materials is achieved.

Description

technical field [0001] The invention relates to a method for preparing a highly gelatinous enzymatic hydrolyzate, more specifically, a method for preparing a highly gelatinous scallop gonad enzymaticallyzate. Background technique [0002] In recent years, with the continuous increase of scallop production, the processing volume of scallops has been increasing year by year. The gonads of scallops contain a large amount of protein components, and the protein content in the male gonads can reach about 87% (by dry weight), which is a good source for developing polypeptides. Contents of the invention [0003] The purpose of the present invention is to develop a method for reprocessing the gonads of the male scallop, improve the utilization rate and added value of the gonads of the scallop, and avoid waste of resources. According to the characteristics of high protein content in the gonads of male scallops, a method of using the gonads of male scallops to prepare an enzymatic h...

Claims

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Application Information

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IPC IPC(8): A23J1/04
CPCA23J1/04
Inventor 吴海涛张萌李毅于翠平孙娜唐越阎佳楠查越刘峻屹
Owner DALIAN POLYTECHNIC UNIVERSITY
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